HRP Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05282
Key Product Details
Species Reactivity
Non-species specific
Applications
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep Serum
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
HRP [Horseradish] (Uniprot: Q42517)
Reactivity Notes
Cross reactivity against HRP from other tissues and species may occur but have not been specifically determined.
Specificity
Cross reactivity against HRP from other tissues and species may occur but have not been specifically determined.
Clonality
Polyclonal
Host
Sheep
Isotype
Serum
Description
This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-sheep serum, purified and partially purified Peroxidase [Horseradish]
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Applications for HRP Antibody - Azide and BSA Free
Application
Recommended Usage
ELISA
1:10000-1:40000
Western Blot
1:1000-1:4000
Application Notes
Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity.
Formulation, Preparation, and Storage
Purification
Delipidation and Defibrination
Reconstitution
Reconstitute with 2.0 ml deionized water (or equivalent)
Formulation
Lyophilized from 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: HRP
Alternate Names
EC 1.11.1.7, Horseradish Peroxidase, HPRC 1, HPRC1, HRPA2, HRPC 2, HRPC2, HRPE5, HRPN, Neutral peroxidase, Peroxidase A2, Peroxidase C1A, Peroxidase C1B, Peroxidase C2, Peroxidase C3, Peroxidase E5, Peroxidase N, PRXC1A, PRXC1B, PRXC2, PRXC3
Additional HRP Products
Product Documents for HRP Antibody - Azide and BSA Free
Product Specific Notices for HRP Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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