HRP Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB600-894
Key Product Details
Species Reactivity
All Species
Applications
Immunohistochemistry, Western Blot, ELISA
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
HRP [Horseradish]
Reactivity Notes
Cross-reacts with HRP from Horseradish. Cross reactivity against HRP from other tissues and species may occur but have not been specifically determined.
Human reactivity reported in scientific literature (PMID: 28983591).
Human reactivity reported in scientific literature (PMID: 28983591).
Localization
Secreted
Specificity
Cross reactivity against HRP from other tissues and species may occur but have not been specifically determined.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Peroxidase [Horseradish]
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for HRP Antibody - BSA Free
![Western Blot: HRP Antibody [NB600-894]](https://resources.bio-techne.com/images/products/HRP-Antibody-Western-Blot-NB600-894-img0001.jpg "Western Blot: HRP Antibody [NB600-894]")
Western Blot: HRP Antibody [NB600-894]
Western Blot: HRP Antibody [NB600-894] - Western Blot Results of Rabbit HRP antibody. Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: HRP (HRP). Primary Antibody: Anti-HRP at 1:100in 0.75%Casein/TBST overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG mx10 DyLight (TM) 649 at 1:5000 in blocking buffer Buffer for 30 mins at RT. Block: 0.75%Casein/TBST for 30 min at RT. Expected MW: ~44kDa.Applications for HRP Antibody - BSA Free
Application
Recommended Usage
ELISA
1:12000-1:54000
Immunohistochemistry
1:10 - 1:500
Western Blot
1:500-1:3000
Application Notes
This product has been tested by western blotting. Specific conditions for reactivity should be optimized by the end user.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Format
BSA Free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: HRP
Alternate Names
EC 1.11.1.7, Horseradish Peroxidase, HPRC 1, HPRC1, HRPA2, HRPC 2, HRPC2, HRPE5, HRPN, Neutral peroxidase, Peroxidase A2, Peroxidase C1A, Peroxidase C1B, Peroxidase C2, Peroxidase C3, Peroxidase E5, Peroxidase N, PRXC1A, PRXC1B, PRXC2, PRXC3
Additional HRP Products
Product Documents for HRP Antibody - BSA Free
Product Specific Notices for HRP Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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