HPV16 E7 Antibody (HL1647) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25510

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25510
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Key Product Details

Species Reactivity

Virus

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Lateral Flow Assay, Sandwich ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1647

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HPV16 E7 Antibodies »

Product Specifications

Immunogen

Full length HPV16 E7 recombinant protein

Reactivity Notes

Human Papillomavirus type 16

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HPV16 E7 Antibody (HL1647) - Azide and BSA Free

HPV16 E7 Antibody (HL1647) - Azide and BSA Free

ELISA: HPV16 E7 Antibody (HL1647) - Azide and BSA Free [NBP3-25510] -

ELISA: HPV16 E7 Antibody (HL1647) - Azide and BSA Free [NBP3-25510] - Sandwich ELISA detection of recombinant Human Papillomavirus type 16 E7 protein, His tag using antibodies as below. Capture: Human Papillomavirus type 16 E7 antibody [HL1821] (NBP3-25511) (5 ug/mL) Detection: HRP-conjugated Human Papillomavirus type 16 E7 antibody [HL1647] (NBP3-25510) (1 ug/mL) Please notice that NBP3-25510 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-25511. Please contact us for custom HRP-conjugated antibody.
HPV16 E7 Antibody (HL1647) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: HPV16 E7 Antibody (HL1647) - Azide and BSA Free [NBP3-25510] -

Immunocytochemistry/Immunofluorescence: HPV16 E7 Antibody (HL1647) - Azide and BSA Free [NBP3-25510] - Human Papilloma virus type 16 E7 antibody [HL1647] detects Human Papilloma virus type 16 E7 protein by immunofluorescent analysis. Sample: Caski cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Human Papilloma virus type 16 E7 stained by Human Papilloma virus type 16 E7 antibody [HL1647] (NBP3-25510) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10um.
HPV16 E7 Antibody (HL1647) - Azide and BSA Free

ELISA: HPV16 E7 Antibody (HL1647) - Azide and BSA Free [NBP3-25510] -

ELISA: HPV16 E7 Antibody (HL1647) - Azide and BSA Free [NBP3-25510] - Sandwich ELISA detection of recombinant Human Papillomavirus type 16 E7 protein, His tag using antibodies as below. Capture: Human Papillomavirus type 16 E7 antibody [HL1647] (NBP3-25510) (5 ug/mL) Detection: HRP-conjugated Human Papillomavirus type 16 E7 antibody [HL1821] (NBP3-25511) (1 ug/mL) Please notice that NBP3-25511 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-25510. Please contact us for custom HRP-conjugated antibody.
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Applications for HPV16 E7 Antibody (HL1647) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Lateral Flow Assay

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HPV16 E7

The HPV E7 proteins are small (HPV16 E7 comprising 98 amino acids, 15kDa), zinc binding phosphoproteins which are localised in the nucleus. They are structurally and functionally similar to the E1A protein of subgenus C adenoviruses. The first 16 amino-terminal amino acids of HPV16 E7 contain a region homologous to a segment of the conserved region 1 (CR1) of the E1A protein of subgenus C adenoviruses. The next domain, up to amino acid 37, is homologous to the entire region 2 (CR2) of E1A. Genetic studies have established that these domains are required for cell transformation in vitro, suggesting similarities in the mechanism of transformation by these viruses. The CR2 homology region contains the LXCXE motif (residues 22-26) involved in binding to the tumour suppressor protein pRb. This sequence is also present in SV40 and polyoma large T antigens. The high risk HPV E7 proteins (of, for example, types 16 and 18) have an approximately ten-fold higher affinity for pRb protein than the low risk HPV E7 proteins (of, for example, type 6). Association of the E7 protein with pRb promotes cell proliferation by the same mechanism as the E1A proteins of adenoviruses and SV40 large T antigen. Recent studies have shown that E7 promotes degradation of Rb family proteins rather than simply inhibiting their function by complex formation. The CR2 region also contains the casein kinase II phosphorylation site (residues 31 and 32). Infection with specific types of HPV has been associated with an increased risk of developing cervical neoplasia. HPV types 6 and 11 have been associated with relatively benign diseases such as genital warts but types 16 and 18 are strongly associated with cervical, vaginal, and vulvar malignancies.

Alternate Names

E7, HPV16 E7, HpV16gp2, Links Transforming protein human papillomavirus 16, Protein E7, Transforming protein, Transforming protein human papillomavirus 16

Additional HPV16 E7 Products

Product Documents for HPV16 E7 Antibody (HL1647) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HPV16 E7 Antibody (HL1647) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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