hnRNP H2 Antibody - BSA Free

Immunohistochemistry-Paraffin: hnRNP H2 Antibody [NBP1-89816]

Immunohistochemistry-Paraffin: hnRNP H2 Antibody [NBP1-89816] - Staining of human testis shows nuclear positivity.

Immunohistochemistry-Paraffin: hnRNP H2 Antibody [NBP1-89816]

Immunohistochemistry-Paraffin: hnRNP H2 Antibody [NBP1-89816] - Staining of human cerebellum shows nuclear positivity in cells in granular layer.

Western Blot: hnRNP H2 Antibody - BSA Free [NBP1-89816]

Analysis in human cell line MCF-7.

Immunocytochemistry/immunofluorescence: hnRNP H2 Antibody [NBP1-89816] -

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Western Blot: hnRNP H2 Antibody [NBP1-89816] -

SMN, PLS3, hnRNP F, & hnRNP H1/2 associate in a large complex. a SMN & PLS3 independently associated with hnRNP H1/2 based on co-immunoprecipitation from HEK293T cells transfected with tagged proteins. b Endogenous PLS3, hnRNP F, & hnRNP H 1/2 associated with SMN in mouse brain extracts, based on immunoprecipitation with antisera against endogenous SMN protein. Arrowheads indicate the band of interest in blots with multiple bands. Heavy & light chain bands are labeled when present. c Endogenous SMN, hnRNP F, & hnRNP H1/2 associated with endogenous PLS3 using the same approach outlined in b. Arrowheads indicate the band of interest in blots with multiple bands. Heavy & light chain bands are labeled when present. IP on endogenous mouse brain extract was done from 3 independent brain extractions & preparations. d Size fractionation studies of whole cell lysates from HEK293T cells showed that actin, SMN, PLS3, & hnRNP F and/or H1/2 proteins co-fractionate in complexes of the indicated size (bracket), although the bulk of hnRNP F/H1/2 protein is in other smaller complexes. HEK293T cells were transfected with PLS3-Flag, & hnRNP F-GFP, endogenous hnRNP H1/2, actin, & SMN were detected using antisera Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32938453), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: hnRNP H2 Antibody [NBP1-89816]

hnRNP-H2-Antibody-Western-Blot-NBP1-89816-img0012.jpg

Western Blot: hnRNP H2 Antibody [NBP1-89816] -

SMN, PLS3, hnRNP F, & hnRNP H1/2 associate in a large complex. a SMN & PLS3 independently associated with hnRNP H1/2 based on co-immunoprecipitation from HEK293T cells transfected with tagged proteins. b Endogenous PLS3, hnRNP F, & hnRNP H 1/2 associated with SMN in mouse brain extracts, based on immunoprecipitation with antisera against endogenous SMN protein. Arrowheads indicate the band of interest in blots with multiple bands. Heavy & light chain bands are labeled when present. c Endogenous SMN, hnRNP F, & hnRNP H1/2 associated with endogenous PLS3 using the same approach outlined in b. Arrowheads indicate the band of interest in blots with multiple bands. Heavy & light chain bands are labeled when present. IP on endogenous mouse brain extract was done from 3 independent brain extractions & preparations. d Size fractionation studies of whole cell lysates from HEK293T cells showed that actin, SMN, PLS3, & hnRNP F and/or H1/2 proteins co-fractionate in complexes of the indicated size (bracket), although the bulk of hnRNP F/H1/2 protein is in other smaller complexes. HEK293T cells were transfected with PLS3-Flag, & hnRNP F-GFP, endogenous hnRNP H1/2, actin, & SMN were detected using antisera Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32938453), licensed under a CC-BY license. Not internally tested by Novus Biologicals.