HNF1 Antibody (HL3229)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-48780
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL3229
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fragment of human HNF1.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit HNF1 Antibody (HL3229) (NBP3-48780) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for HNF1 Antibody (HL3229)
Immunocytochemistry/ Immunofluorescence: HNF1 Antibody (HL3229) [NBP3-48780] -
HNF1 antibody [HL3229] detects HNF1 protein by immunofluorescent analysis.Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HNF1 stained by HNF1 antibody [HL3229] (NBP3-48780) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Western Blot: HNF1 Antibody (HL3229) [NBP3-48780] -
Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 antibody [HL3229] (NBP3-48780) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: HNF1 Antibody (HL3229) [NBP3-48780] -
HNF1 antibody [HL3229] detects HNF1 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.HNF1 stained by HNF1 antibody [HL3229] (NBP3-48780) diluted at 1:100.Antigen Retrieval: Tris-EDTA-Citrate acid buffer, pH 7.8, 15 minCorresponding RNA expression data for the same tissues are based on NCBI.
Applications for HNF1 Antibody (HL3229)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
1:500-1:10000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HNF1
Alternate Names
HNF1 homeobox A, IDDM20, LFB1transcription factor 1, hepatic; LF-B1, hepatic nuclear factor (HNF1), albuminproximal factor, MODY3, TCF1hepatic, Transcription factor 1
Gene Symbol
HNF1A
Additional HNF1 Products
Product Documents for HNF1 Antibody (HL3229)
Product Specific Notices for HNF1 Antibody (HL3229)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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