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HNF-3 beta/FoxA2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-57623

Novus Biologicals, part of Bio-Techne
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NBP2-57623
NBP2-57623-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: SHHHHQPHKMDLKAYEQVMHYPGYGSPMPGSLAMGPVTNKTGLDASPLAADTS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit HNF-3 beta/FoxA2 Antibody - BSA Free (NBP2-57623) is a polyclonal antibody validated for use in ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for HNF-3 beta/FoxA2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: HNF-3 beta/FoxA2 Antibody [NBP2-57623]

Immunocytochemistry/ Immunofluorescence: HNF-3 beta/FoxA2 Antibody [NBP2-57623]

Immunocytochemistry/Immunofluorescence: HNF-3 beta/FoxA2 Antibody [NBP2-57623] - Staining of human cell line A549 shows localization to nucleoplasm & cell junctions. Antibody staining is shown in green.
HNF-3 beta/FoxA2 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: HNF-3 beta/FoxA2 Antibody - BSA Free [NBP2-57623]

Chromatin Immunoprecipitation-exo-Seq: HNF-3 beta/FoxA2 Antibody - BSA Free [NBP2-57623]

ChIP-Exo-Seq composite graph for Anti-FOXA2 (NBP2-57623) tested in HepG2 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for HNF-3 beta/FoxA2 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HNF-3 beta/FoxA2

FOXA2 encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporadic cases of maturity-onset diabetes of the young. Two transcript variants encoding the same protein have been identified for this gene.

Long Name

Hepatocyte Nuclear Factor-3, beta

Alternate Names

FoxA2, HNF3 beta

Gene Symbol

FOXA2

Additional HNF-3 beta/FoxA2 Products

Product Documents for HNF-3 beta/FoxA2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HNF-3 beta/FoxA2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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