HMGB1/HMG-1 Antibody (19N10B7) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27396

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NBP2-27396SS
NBP2-27396
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Bovine (99%), Canine (100%), Chicken (91%), Chinese Hamster (99%), Equine (100%), Porcine (99%), Primate (100%), Rat (99%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Simple Western, Immunoprecipitation, CyTOF-ready

Cited:

Immunoprecipitation, In vivo assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 19N10B7

Format

BSA Free

Concentration

1.0 mg/ml

View all HMGB1/HMG-1 Antibodies »

Product Specifications

Immunogen

Full-length recombinant human HMGB1 protein

Reactivity Notes

Based upon immunogen sequence similarity, this antibody is predicted to react with Rat (99%), Bovine (99%), Porcine/Pig (99%), Chinese Hamster (99%), Chicken (91%), Canine/Dog (100%), Primate/Monkey (100%) and Equine/Horse (100%). Mouse reactivity reported in a verified customer review.

Localization

Nucleus (very high), Cytoplasm (very low)

Specificity

This antibody recognizes both A box domain (amino acids 9-79) and full length HMGB1 protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for HMGB1/HMG-1 Antibody (19N10B7) - BSA Free

Western Blot: HMGB1/HMG-1 Antibody (19N10B7)BSA Free [NBP2-27396]

Western Blot: HMGB1/HMG-1 Antibody (19N10B7)BSA Free [NBP2-27396]

Western Blot: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - Hepatocyte protein lysate at 1:1000 4C overnight. Image from verified customer review.
Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) - BSA Free [NBP2-27396]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) - BSA Free [NBP2-27396]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - Formalin-fixed, paraffin-embedded human liver tissue stained with HMGB1 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Flow Cytometry: HMGB1/HMG-1 Antibody (19N10B7) - BSA Free [NBP2-27396]

Flow Cytometry: HMGB1/HMG-1 Antibody (19N10B7) - BSA Free [NBP2-27396]

Flow Cytometry: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - Intracellular analysis using HMGB1 antibody. Human Jurkat cells were probed using 0.5 ug of HMGB1 antibody (red) and 0.5 ug of isotype control antibody (green).
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Applications for HMGB1/HMG-1 Antibody (19N10B7) - BSA Free

Application
Recommended Usage

Flow Cytometry

0.5ug / 5x10^5cells

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Immunoprecipitation

reported in scientific literature (PMID 23696858)

Simple Western

1:50

Western Blot

1-3 ug/ml. Use reported by customer review
Application Notes
In Simple Western only 10 - 15 ul of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 1.0 mg/mL, separated by Size, antibody dilution of 1:50, apparent MW was 34 kDa. This antibody is CyTOF ready.

Reviewed Applications

Read 1 review rated 5 using NBP2-27396 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HMGB1/HMG-1

HMGB1 (high mobility group protein B1) belongs to the HMGB family and contains two HMG box DNA-binding domains. It is a highly conserved, ubiquitous protein present in the nuclei and cytoplasm of nearly all cell types, and is a necessary and sufficient mediator of inflammation during sterile and infection-associated responses. HMGB1 also act as DNA nuclear binding protein that has recently been shown to be an early trigger of sterile inflammation in animal models of trauma-hemorrhage via the activation of the Toll-like receptor 4 (TLR4) and the receptor for the advanced glycation endproducts (RAGE). It is reported that the level of HMGB1 is elevated during sterile tissue injury, infection, lethal endotoxemia or sepsis, collagen-induced arthritis, and ischemia-reperfusion induced tissue injury.

Long Name

High Mobility Group Protein 1

Alternate Names

HMG-1, HMG1

Gene Symbol

HMGB1

Additional HMGB1/HMG-1 Products

Product Documents for HMGB1/HMG-1 Antibody (19N10B7) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HMGB1/HMG-1 Antibody (19N10B7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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