HLA G Antibody (MEM-G/9) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB500-314
![Flow Cytometry: HLA G Antibody (MEM-G/9) - BSA Free [NB500-314]](https://resources.bio-techne.com/images/products/HLA-G-Antibody-MEM-G-9-Flow-Cytometry-NB500-314-img0011.jpg "Flow Cytometry: HLA G Antibody (MEM-G/9) - BSA Free [NB500-314]")
![Immunoprecipitation: HLA G Antibody (MEM-G/9) - BSA Free [NB500-314]](https://resources.bio-techne.com/images/products/HLA-G-Antibody-MEM-G-9-Immunoprecipitation-NB500-314-img0009.jpg "Immunoprecipitation: HLA G Antibody (MEM-G/9) - BSA Free [NB500-314]")
![Immunocytochemistry/ Immunofluorescence: HLA G Antibody (MEM-G/9) - BSA Free [NB500-314]](https://resources.bio-techne.com/images/products/HLA-G-Antibody-MEM-G-9-Immunocytochemistry-Immunofluorescence-NB500-314-img0006.jpg "Immunocytochemistry/ Immunofluorescence: HLA G Antibody (MEM-G/9) - BSA Free [NB500-314]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse (Negative)
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready
Cited:
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # MEM-G/9
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant human HLA G Antibody (MEM-G/9) refolded with beta2-microglobulin and peptide (NM_002127.5)
Epitope
aa 16-25 of human p53
Localization
Cell Membrane
Specificity
The antibody MEM-G/9 reacts with native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. Reactivity with HLA-G3 was also reported. The antibody MEM-G/9 is standard reagent thoroughly validated during 3rd International Conference on HLA-G (Paris, 2003).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for HLA G Antibody (MEM-G/9) - BSA Free
Immunocytochemistry/ Immunofluorescence: HLA G Antibody (MEM-G/9) - BSA Free [NB500-314]
Immunocytochemistry/Immunofluorescence: HLA G Antibody (MEM-G/9) [NB500-314] - Staining of HLA-G1 transfectants (LCL-HLA-G1) using Alexa Fluor 488 conjugated anti-human HLA-G (MEM-G/9).![Flow Cytometry: HLA G Antibody (MEM-G/9) - BSA Free [NB500-314]](https://resources.bio-techne.com/images/products/HLA-G-Antibody-MEM-G-9-Flow-Cytometry-NB500-314-img0012.jpg "Flow Cytometry: HLA G Antibody (MEM-G/9) - BSA Free [NB500-314]")
Flow Cytometry: HLA G Antibody (MEM-G/9) - BSA Free [NB500-314]
Flow Cytometry: HLA G Antibody (MEM-G/9) [NB500-314] - Surface staining of HLA-G1 transfectants (LCL-HLA-G1) using anti-HLA-G (MEM-G/9) PE.![Flow Cytometry: HLA G Antibody (MEM-G/9) - BSA Free [NB500-314]](https://resources.bio-techne.com/images/products/HLA-G-Antibody-MEM-G-9-Flow-Cytometry-NB500-314-img0010.jpg "Flow Cytometry: HLA G Antibody (MEM-G/9) - BSA Free [NB500-314]")
Flow Cytometry: HLA G Antibody (MEM-G/9) - BSA Free [NB500-314]
Flow Cytometry: HLA G Antibody (MEM-G/9) [NB500-314] - Analysis of APC conjugate of NB500-314. Double surface staining of HLA-G1 transfectants (viable cells gate) using anti-human HLA-G (MEM-G/9) APC and anti-human HLA-G (MEM-G/11) FITC.Applications for HLA G Antibody (MEM-G/9) - BSA Free
Application
Recommended Usage
ELISA
1:2000
Flow Cytometry
0.3-4 ug/ml
Immunocytochemistry/ Immunofluorescence
2-5 ug/ml
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:200
Immunoprecipitation
1:50
Western Blot
1:100-1:2000
Application Notes
ELISA: The antibody MEM-G/9 has been tested as the capture antibody in a sandwich ELISA for analysis of human HLA-G in combination with antibody B2M-01 or with antibody W6/32. Coating antibody (10 ug/ml), detection antibody (biotin or peroxidase conjugate, 1 ug/ml). Purity is > 95% (by SDS-PAGE). Use in western blot reported in scientific literature ( PMID 24054889). This antibody is CyTOF ready.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
Phosphate buffered saline (PBS), pH 7.4
Format
BSA Free
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: HLA-G
Discovered in cytotrophoblasts, HLA-G is involved in fetal maternal immune tolerance and some studies have linked its downregulation with severe preeclampsia (3). HLA-G mediated immune suppression works by impeding cell proliferation, differentiation, cytotoxicity, cytokine secretion and chemotaxis; and activation of regulatory cells and MDSCs or M2 type macrophage. HLA-G is constitutively expressed in immune-privileged sites such as pancreatic islets, mesenchymal stem cells (MSCs) and the cornea. Upregulation of HLA-G occurs in pathological states including cancer, allo-transplantations, viral infections, autoimmune and inflammatory diseases (4). In cancer, HLA-G expression is induced by hypoxia and has been correlated with advanced tumor stage, tumor metastasis, and poor therapeutic response and survival. HLA-G is an attractive tumor associated-antigen (TAA) for immunotherapy and is considered a major immune checkpoint (ICP).
References
1. Loustau, M., Anna, F., Drean, R., Lecomte, M., Langlade-Demoyen, P., & Caumartin, J. (2020). HLA-G Neo-Expression on Tumors. Front Immunol, 11:1685. PMID: 32922387
2. Lin A, Yan WH. (2018) Heterogeneity of HLA-G Expression in Cancers: Facing the Challenges. Front Immunol. 9:2164. PMID: 30319626
3. Djurisic S, Hviid TV. (2014) HLA Class Ib Molecules and Immune Cells in Pregnancy and Preeclampsia. Front Immunol. 5:652. PMID: 25566263
4. Lila N, Rouas-Freiss N, Dausset J, Carpentier A, Carosella ED. (2001) Soluble HLA-G protein secreted by allo-specific CD4+ T cells suppresses the allo-proliferative response: a CD4+ T cell regulatory mechanism. Proc Natl Acad Sci U S A. 98(21):12150-12155. PMID: 11572934
Long Name
Major Histocompatibility Complex, Class I, G
Alternate Names
HLA G, HLA-6.0, HLAG, MHC Class I Antigen G, MHC-G
Gene Symbol
HLA-G
Additional HLA-G Products
Product Documents for HLA G Antibody (MEM-G/9) - BSA Free
Product Specific Notices for HLA G Antibody (MEM-G/9) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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