HLA Class I Antibody (W6/32) - Low Endotoxin
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-09091
Key Product Details
Species Reactivity
Human, Bovine, Feline, Primate, Rabbit (Negative)
Applications
CyTOF-ready, ELISA, Flow Cytometry, Functional, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # W6/32
Format
Low Endotoxin
Concentration
1 mg/ml
Product Specifications
Immunogen
Membrane of human tonsil cells
Reactivity Notes
This antibody does not react with: Rabbit
Specificity
The antibody W6/32 recognises an extracellular epitope of MHC Class I molecules (MHC Class Ia) that are expressed on the surface of all human nucleated cell types. The antibody W6/32 is a valuable reagent for analysing variations in HLA class I expression in different disease states e.g. liver disease, muscular dystrophy, inflammatory myopathy and other neuromuscular disorders. This antibody W6/32 is also suitable as a positive control for HLA tissue typing and crossmatching.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Endotoxin Level
Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.
Scientific Data Images for HLA Class I Antibody (W6/32) - Low Endotoxin
![HLA Class I Antibody (W6/32) - Low Endotoxin, Azide and BSA Free Flow Cytometry: HLA Class I Antibody (W6/32) - Low Endotoxin, Azide and BSA Free [NBP3-09091]](https://resources.bio-techne.com/images/products/nbp3-09091_mouse-monoclonal-hla-class-i-antibody-w6-32-low-endotoxin-azide-and-bsa-free-13120241659356.jpg "Flow Cytometry: HLA Class I Antibody (W6/32) - Low Endotoxin, Azide and BSA Free [NBP3-09091]")
Flow Cytometry: HLA Class I Antibody (W6/32) - Low Endotoxin, Azide and BSA Free [NBP3-09091]
Separation of leukocytes stained using anti-HLA Class I (W6/32) purified antibody (concentration in sample 4 ug/ml, GAM APC, red-filled) from leukocytes unstained by primary antibody (GAM APC, black-dashed) in Analysis (surface staining).Applications for HLA Class I Antibody (W6/32) - Low Endotoxin
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Functional
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
Phosphate buffered saline (PBS), pH 7.4
Format
Low Endotoxin
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HLA Class I
Long Name
Class I Human Leukocyte Antigen
Alternate Names
HLA-A, HLA-ABC
Gene Symbol
HLA-A
Additional HLA Class I Products
Product Documents for HLA Class I Antibody (W6/32) - Low Endotoxin
Product Specific Notices for HLA Class I Antibody (W6/32) - Low Endotoxin
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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