Histone H4 [ac Lys12] Antibody - BSA Free

Western Blot: Histone H4 [ac Lys12] Antibody [NBP2-59266]

Western Blot: Histone H4 [ac Lys12] Antibody [NBP2-59266] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H4K12ac. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right, the marker (in kDa) is shown on the left.

Immunocytochemistry/ Immunofluorescence: Histone H4 [ac Lys12] Antibody [NBP2-59266]

Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys12] Antibody [NBP2-59266] - HeLa cells were stained with the antibody against H4K12ac and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H4K12ac antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

Chromatin Immunoprecipitation: Histone H4 [ac Lys12] Antibody [NBP2-59266] - ChIP assays were performed using human HeLa cells, the antibody against H4K12ac and optimized PCR primer sets for qPCR. ChIP was performed with the ChIP-seq kit on sheared chromatin from 1,000,000 cells. A titration of the antibody consisting of 0.5, 1, 2 and 5 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for promoter of the active GAPDH and EIF4A2 genes, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat region used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).