Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody

Western Blot: Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody [NBP2-16848]

Western Blot: Histone H4 Antibody [NBP2-16848] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: BCL-1 15% SDS PAGE diluted at 1:10000

Western Blot: Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody [NBP2-16848]

Western Blot: Histone H4 Antibody [NBP2-16848] - Sample (30 ug of whole cell lysate) A: HeLa 15% SDS PAGE diluted at 1:10000

Dot Blot: Histone H4 Antibody [NBP2-16848] - Peptide samples (0.1 ug) were spotted onto positively charged nylon membrane and blotted with Histone H4 (acetyl Lys6, Lys9, Lys13, Lys17) antibody Histone H4 at different dilution as indicated. A: Peptide samples of Histone H4 (acetyl Lys6, Lys9, Lys13, Lys17). B: Peptide samples of Histone H4

Western Blot: Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody [NBP2-16848] -

Reduced histone levels in senescent cells is induced in vitro by different means, & in vivo from aged donors. (a) Phase-contrast images of primary fibroblasts induced into senescence by chronic gamma-radiation, oncogene over-expression or exhaustive replication (replicative senescence), & DNA damage from a single acute 4 Gy X-ray dose at an early time-point (1 hour) as a control for DNA damage without senescence. Scale bars 200 µm. (b) Western immunoblot analyses of histones in fibroblasts described in (a). (c) Histone levels in dermal fibroblasts isolated from human neonatal (age 0, donors a & b) & adult donors. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32042076), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody [NBP2-16848] -

Chronic gamma-radiation reduces histone levels. (a) Western blot analyses of histone H2AX & gammaH2AX in primary fibroblasts exposed to various dose-rates of chronic gamma-radiation for 7 days. Cells irradiated with a single acute dose of 4 Gy X-ray were included as control. (b) Effect of chronic gamma-irradiation on H2AX levels in three different isogenic primary cell types from a different donor to that used in (a) at the same dose rates as in (a). (c) Immunoblots of other histones in chronically irradiated primary fibroblasts. (d) All significant histone level changes detected by SILAC LC-MS/MS protein analyses of samples from primary fibroblasts exposed or mock-exposed to chronic gamma-radiation for 7 days. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32042076), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody [NBP2-16848] -

Reduced histone levels in senescent cells is induced in vitro by different means, & in vivo from aged donors. (a) Phase-contrast images of primary fibroblasts induced into senescence by chronic gamma-radiation, oncogene over-expression or exhaustive replication (replicative senescence), & DNA damage from a single acute 4 Gy X-ray dose at an early time-point (1 hour) as a control for DNA damage without senescence. Scale bars 200 µm. (b) Western immunoblot analyses of histones in fibroblasts described in (a). (c) Histone levels in dermal fibroblasts isolated from human neonatal (age 0, donors a & b) & adult donors. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32042076), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody [NBP2-16848] -

Western Blot: Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody [NBP2-16848] - Sample (30 ug of whole cell lysate)
A: HeLa
15% SDS PAGE
NBP2-16848 diluted at 1:10000

Immunocytochemistry/ Immunofluorescence: Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody [NBP2-16848] -

Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody detects Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) protein stained by Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody (NBP2-16848) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 um.