Histone H4 [p Ser1] Antibody (RM194) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26008
Recombinant Monoclonal Antibody
![Histone H4 [p Ser1] Antibody (RM194) - Azide and BSA Free](https://resources.bio-techne.com/images/products/nbp3-26008_rabbit-histone-h4-mab-rm194-azide-and-bsa-free-246202413265.png "Western Blot: Histone H4 [p Ser1] Antibody (RM194) - Azide and BSA Free [NBP3-26008] -")
Key Product Details
Species Reactivity
Human, Vertebrate
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Multiplex Immunoassay, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM194
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
A phospho-peptide corresponding to Histone H4 [p Ser1]
Specificity
This antibody reacts to Histone H4 phosphorylated at Serine 1. The reactivity is not affected by neighbor Arginine 3 modification. No cross reactivity with Histone H2A phosphorylated at Serine 1.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Histone H4 [p Ser1] Antibody (RM194) - Azide and BSA Free
Western Blot: Histone H4 [p Ser1] Antibody (RM194) - Azide and BSA Free [NBP3-26008] -
Western Blot: Histone H4 [p Ser1] Antibody (RM194) - Azide and BSA Free [NBP3-26008] - Western Blot of (1) acid extracts of HeLa cells; (2) recombinant Histone H4, using RM194 at 0.5 ug/mL, showed a band of Histone H4 phosphorylated at Serine 1 in HeLa cells.![Histone H4 [p Ser1] Antibody (RM194) - Azide and BSA Free](https://resources.bio-techne.com/images/products/nbp3-26008_rabbit-histone-h4-mab-rm194-azide-and-bsa-free-24620241332410.png "Multiplex Immunoassay: Histone H4 [p Ser1] Antibody (RM194) - Azide and BSA Free [NBP3-26008] -")
Multiplex Immunoassay: Histone H4 [p Ser1] Antibody (RM194) - Azide and BSA Free [NBP3-26008] -
Multiplex Immunoassay: Histone H4 [p Ser1] Antibody (RM194) - Azide and BSA Free [NBP3-26008] - RM194 specifically reacts to Histone H4 phosphorylated at Serine 1 (H4S1p). The reactivity is not affected by neighboring Arginine 3 modifications (H4S1pR3me1 and H4S1pR3me2). No cross reactivity with Histone H2A phosphorylated at Serine 1 (H2AS1p).![Histone H4 [p Ser1] Antibody (RM194) - Azide and BSA Free](https://resources.bio-techne.com/images/products/nbp3-26008_rabbit-histone-h4-mab-rm194-azide-and-bsa-free-24620241332413.png "Immunocytochemistry/ Immunofluorescence: Histone H4 [p Ser1] Antibody (RM194) - Azide and BSA Free [NBP3-26008] -")
Immunocytochemistry/ Immunofluorescence: Histone H4 [p Ser1] Antibody (RM194) - Azide and BSA Free [NBP3-26008] -
Immunocytochemistry/ Immunofluorescence: Histone H4 [p Ser1] Antibody (RM194) - Azide and BSA Free [NBP3-26008] - Immunocytochemistry of HeLa cells, using Clone RM194 (red). Actin filaments have been labeled with fluorescein phalloidin (green).Applications for Histone H4 [p Ser1] Antibody (RM194) - Azide and BSA Free
Application
Recommended Usage
ELISA
0.2 ug/mL - 1 ug/mL
Immunocytochemistry/ Immunofluorescence
1 ug/ml - 2 ug/ml
Multiplex Immunoassay
0.1 ug/ml - 1 ug/ml
Western Blot
0.5 ug/ml-2 ug/ml
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Histone H4
Alternate Names
H4, H4/M, H4FM, H4M, HIST1H4I, HIST4H4, Histone Cluster 1 H4, Histone Cluster 1 H4i, Histone Cluster 4 H4
Gene Symbol
H4C16
Additional Histone H4 Products
Product Documents for Histone H4 [p Ser1] Antibody (RM194) - Azide and BSA Free
Product Specific Notices for Histone H4 [p Ser1] Antibody (RM194) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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