Histone H3.3 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24697
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Bovine, Chicken, Drosophila, Equine, Opossum, Zebrafish
Cited:
Avian - Chicken
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunohistochemistry, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A portion of amino acids 100-136 of human Histone H3.3 was used as the immunogen for the antibody.
Reactivity Notes
Ortangutan (100%), Salmon (100%).
Specificity
100 % homologous in human (Histone H3.3A, B, and C).
Marker
Marker of Transcriptionally Active Chromatin
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Histone H3.3 Antibody - BSA Free
![Western Blot: Histone H3.3 Antibody [NBP2-24697]](https://resources.bio-techne.com/images/products/Histone-H3.3-Antibody-Western-Blot-NBP2-24697-img0003.jpg "Western Blot: Histone H3.3 Antibody [NBP2-24697]")
Western Blot: Histone H3.3 Antibody [NBP2-24697]
Western Blot: Histone H3.3 Antibody [NBP2-24697] - Total protein from human HeLa and A431 cells and mouse Neuro2A cells was separated on a 4-15% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/ml anti-Histone H3.3 in blocking buffer with an anti-rabbit HRP secondary antibody using chemiluminescence.![Immunohistochemistry-Paraffin: Histone H3.3 Antibody [NBP2-24697]](https://resources.bio-techne.com/images/products/Histone-H3.3-Antibody-Immunohistochemistry-Paraffin-NBP2-24697-img0002.jpg "Immunohistochemistry-Paraffin: Histone H3.3 Antibody [NBP2-24697]")
Immunohistochemistry-Paraffin: Histone H3.3 Antibody [NBP2-24697]
Immunohistochemistry-Paraffin: Histone H3.3 Antibody [NBP2-24697] - Human small intestine stained with Histone H3.3 antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.Applications for Histone H3.3 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
5-10 ug/ml
Western Blot
0.25-0.5 ug/ml
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Histone H3.3
Alternate Names
H3.3A, H3F3, H3F3A, Histone H3.3, Histone H3.3A, Histone H3.3B, MGC87782, MGC87783
Gene Symbol
H3-3A
Additional Histone H3.3 Products
Product Documents for Histone H3.3 Antibody - BSA Free
Product Specific Notices for Histone H3.3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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