Histone H3.3 [p Ser31] Antibody (23GB6015)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50449

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB6015

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Histone H3.3 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Phospho-Histone H3.3 (S31) (Uniprot: P84243)

Modification

p Ser31

Specificity

This antibody detects endogenous levels of H3-3A only when phosphorylated at S31

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone H3.3 [p Ser31] Antibody (23GB6015)

Histone H3.3 [p Ser31] Antibody (23GB6015)

Immunocytochemistry/ Immunofluorescence: Histone H3.3 [p Ser31] Antibody (23GB6015)[NBP3-50449] -

Immunocytochemical staining of HepG2 cells with Histone H3.3 antibody (1:1,000). Nuclei were stained blue with DAPI; Histone H3.3 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
Histone H3.3 [p Ser31] Antibody (23GB6015)

Flow Cytometry: Histone H3.3 [p Ser31] Antibody (23GB6015)[NBP3-50449] -

Flow cytometric analysis of Histone H3.3expression in HepG2 cells using Histone H3.3 antibody (1:2,000). Green, isotype control; red, Phospho-Histone H3.3 (S31).
Histone H3.3 [p Ser31] Antibody (23GB6015)

Western Blot: Histone H3.3 [p Ser31] Antibody (23GB6015)[NBP3-50449] -

Western blotting analysis using Histone H3.3 (S31) antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for Histone H3.3 [p Ser31] Antibody (23GB6015)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histone H3.3

Alternate Names

H3.3A, H3F3, H3F3A, Histone H3.3, Histone H3.3A, Histone H3.3B, MGC87782, MGC87783

Gene Symbol

H3-3A

Additional Histone H3.3 Products

Product Documents for Histone H3.3 [p Ser31] Antibody (23GB6015)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Histone H3.3 [p Ser31] Antibody (23GB6015)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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