Histone H2AX Antibody (4Z3S8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15378
Recombinant Monoclonal Antibody
![Immunohistochemistry-Paraffin: Histone H2AX Antibody (4Z3S8) [NBP3-15378]](https://resources.bio-techne.com/images/products/Histone-H2AX-Antibody-4Z3S8-Immunohistochemistry-Paraffin-NBP3-15378-img0002.jpg "Immunohistochemistry-Paraffin: Histone H2AX Antibody (4Z3S8) [NBP3-15378]")
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Chromatin Immunoprecipitation (ChIP)
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 4Z3S8 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histone H2AX (P16104). MSGRGKTGGKARAKAKSRSSRAGLQFPVGRVHRLLRKGHYAERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGG
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Histone H2AX Antibody (4Z3S8)
![Western Blot: Histone H2AX Antibody (4Z3S8) [NBP3-15378]](https://resources.bio-techne.com/images/products/Histone-H2AX-Antibody-4Z3S8-Western-Blot-NBP3-15378-img0004.jpg "Western Blot: Histone H2AX Antibody (4Z3S8) [NBP3-15378]")
Western Blot: Histone H2AX Antibody (4Z3S8) [NBP3-15378]
Western Blot: Histone H2AX Antibody (4Z3S8) [NBP3-15378] - Western blot analysis of extracts of various cell lines, using Histone H2AX Rabbit mAb (NBP3-15378) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.![Immunohistochemistry-Paraffin: Histone H2AX Antibody (4Z3S8) [NBP3-15378]](https://resources.bio-techne.com/images/products/Histone-H2AX-Antibody-4Z3S8-Immunohistochemistry-Paraffin-NBP3-15378-img0003.jpg "Immunohistochemistry-Paraffin: Histone H2AX Antibody (4Z3S8) [NBP3-15378]")
Immunohistochemistry-Paraffin: Histone H2AX Antibody (4Z3S8) [NBP3-15378]
Immunohistochemistry-Paraffin: Histone H2AX Antibody (4Z3S8) [NBP3-15378] - Immunohistochemistry of paraffin-embedded Mouse brain using Histone H2AX antibody (NBP3-15378) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.![Immunohistochemistry-Paraffin: Histone H2AX Antibody (4Z3S8) [NBP3-15378]](https://resources.bio-techne.com/images/products/Histone-H2AX-Antibody-4Z3S8-Immunohistochemistry-Paraffin-NBP3-15378-img0001.jpg "Immunohistochemistry-Paraffin: Histone H2AX Antibody (4Z3S8) [NBP3-15378]")
Immunohistochemistry-Paraffin: Histone H2AX Antibody (4Z3S8) [NBP3-15378]
Immunohistochemistry-Paraffin: Histone H2AX Antibody (4Z3S8) [NBP3-15378] - Immunohistochemistry of paraffin-embedded Human liver cancer using Histone H2AX antibody (NBP3-15378) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Applications for Histone H2AX Antibody (4Z3S8)
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
1:50-1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Histone H2AX
Histone H2AX is one of a number of core histone proteins. In the cellular response to genotoxic insults, ATM and related protein kinases phosphorylate the carboxyl-terminal tail of the H2AX protein (gamma-H2AX). gamma-H2AX marks the site of damage and provides a nucleation site for the formation of damage response and repair complexes. BRCA1 carboxyl terminal (BRCT) domain-containing proteins are recruited to gamma-H2AX to create a protein scaffold for further assembly of signaling complexes.
Alternate Names
H2AFX
Gene Symbol
H2AX
Additional Histone H2AX Products
Product Documents for Histone H2AX Antibody (4Z3S8)
Product Specific Notices for Histone H2AX Antibody (4Z3S8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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