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Histone H1.3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-48449

Novus Biologicals, part of Bio-Techne
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NBP3-48449

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Histone H1.3. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone H1.3 Antibody

Histone H1.3 Antibody

Immunohistochemistry: Histone H1.3 Antibody [NBP3-48449] -

Histone H1.3 antibody detects Histone H1.3 protein at nucleus on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
Histone H1.3 antibody (NBP3-48449) dilution: 1:500.

Antigen Retrieval: (EDTA based, pH 8.0) buffer, 15min
Histone H1.3 Antibody

Immunocytochemistry/ Immunofluorescence: Histone H1.3 Antibody [NBP3-48449] -

Histone H1.3 antibody detects Histone H1.3 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H1.3 protein stained by Histone H1.3 antibody (NBP3-48449) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Scale bar = 10 um.
Histone H1.3 Antibody

Immunohistochemistry: Histone H1.3 Antibody [NBP3-48449] -

Histone H1.3 antibody detects Histone H1.3 protein at nucleus on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
Histone H1.3 antibody (NBP3-48449) dilution: 1:500.


Antigen Retrieval: (EDTA based, pH 8.0) buffer, 15min

Applications for Histone H1.3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone H1.3

Histones are nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber as they play a critical role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. The histone H1.3 protein is encoded by the HIST1H1D gene and is 221 amino acids long at 22 kDA. H1 histones are critical for the condensation of nucleosome chains into higher order structures. HIST1H1D participates in PKA signaling, cell cycle start of DNA replication in early S phase, apoptosis, and histone modification and is known to interact with genes LMNA, EP300, SUMO1, NOP56 and YWHAQ. Researchers have investigated its role in treacher collins syndrome, meningioma, typhoid fever, and crohn's disease.

Alternate Names

H1 histone family, member 3, H1.3, H1F3H1D, histone 1, H1d, histone cluster 1, H1d, histone H1.3, Histone H1c, MGC138176

Gene Symbol

H1-3

Additional Histone H1.3 Products

Product Documents for Histone H1.3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Histone H1.3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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