Histone Deacetylase 4/HDAC4 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22151

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Primate

Cited:

Human, Mouse, Transgenic Mouse

Predicted:

Chicken (94%), Zebrafish (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all Histone Deacetylase 4/HDAC4 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a C-terminal portion of the human HDAC4 protein (between residues 1000-1084). [UniProt P56524]

Localization

Nucleus and cytoplasm.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Histone Deacetylase 4/HDAC4 Antibody - BSA Free (NBP2-22151) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and Simple Western. Anti-Histone Deacetylase 4/HDAC4 Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Histone Deacetylase 4/HDAC4 Antibody - BSA Free

Western Blot: Histone Deacetylase 4/HDAC4 AntibodyBSA Free [NBP2-22151]

Western Blot: Histone Deacetylase 4/HDAC4 AntibodyBSA Free [NBP2-22151]

Histone-Deacetylase-4-HDAC4-Antibody-Western-Blot-NBP2-22151-img0006.jpg
Immunocytochemistry/ Immunofluorescence: Histone Deacetylase 4/HDAC4 Antibody - BSA Free [NBP2-22151]

Immunocytochemistry/ Immunofluorescence: Histone Deacetylase 4/HDAC4 Antibody - BSA Free [NBP2-22151]

Immunocytochemistry/Immunofluorescence: Histone Deacetylase 4/HDAC4 Antibody [NBP2-22151] - HDAC4 antibody was tested in HeLa cells at a 1:100 dilution against DyLight 488 (Green) using a 40X objective. Alpha tubulin and nuclei were counterstained against DyLight 568 (Red) and DAPI (Blue), respectively.
Immunohistochemistry: Histone Deacetylase 4/HDAC4 Antibody - BSA Free [NBP2-22151]

Immunohistochemistry: Histone Deacetylase 4/HDAC4 Antibody - BSA Free [NBP2-22151]

Histone-Deacetylase-4-HDAC4-Antibody-Immunohistochemistry-NBP2-22151-img0007.jpg
View 3 more images

Applications for Histone Deacetylase 4/HDAC4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500 - 1:1000

Immunohistochemistry

1:300

Immunohistochemistry-Paraffin

1:300

Simple Western

1:100

Western Blot

1.0 ug/mL
Application Notes
This HDAC4 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry on paraffin embedded sections. In Western blot a band was observed approx. 130 kDa. In ICC/IF nuclear and cytoplasmic staining was observed in HeLa cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in NIH-3T3 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:100. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone Deacetylase 4/HDAC4

Histone deacetylase 4 (HDAC4), also known as HD4, KIAA0288, AHO3, BDMR, HDACA, and HA6116, is crucial for transcription regulation, cell cycle progression and developmental events. HDAC4 also plays a critical role in the deacetylation of lysine residues in the core histones, which results in the production of a tag for epigenetic repression. HDAC4 is a member of the class II histone deacetylase/AcuC/AphA family and has been linked to nucleosomal condensation. The HDAC4 protein is expressed ubiquitously throughout the body and can be found in both the nucleus and cytoplasm. HDAC4 gathers in the nuclei of myotubes during muscle cell differentiation, which indicates HDAC4 plays a role in muscle differentiation. HDAC4 is exported to the cytoplasm based on the interactions with a 14-3-3 chaperone protein and its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4 and SIK1. Mutations in HDAC4 can cause brachydactyly-mental retardation syndrome (BDMR), which resembles Albright hereditary osteodystrophy. Recent research has found that HDAC4 regulates HIF-1, which influences cancer cell production and reaction to hypoxia making HDAC4 a possible protein of interest in cancer treatment (PMID: 21917920). Further research has found that HDAC4 affects the growth of cancerous cells in the prostate (PMID: 19013255). Other areas of research, involving knock-out studies, have found a link between HDAC4 and long term memory formation (PMID: 21542139).

Long Name

Histone Deacetylase 4

Alternate Names

HDAC4

Entrez Gene IDs

9759 (Human); 208727 (Mouse)

Gene Symbol

HDAC4

UniProt

P56524 (Human)

Additional Histone Deacetylase 4/HDAC4 Products

Product Documents for Histone Deacetylase 4/HDAC4 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Histone Deacetylase 4/HDAC4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.