Histone Deacetylase 4/HDAC4 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56341

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NB100-56341
NB100-56341SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Chicken (100%), Primate (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

Western Blot, Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all Histone Deacetylase 4/HDAC4 Antibodies »

Product Specifications

Immunogen

This antibody was generated by immunizing rabbits with a synthetic peptide corresponding to amino acids 194-209 of human HDAC4.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29380950)..

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone Deacetylase 4/HDAC4 Antibody - BSA Free

Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NB100-56341]

Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NB100-56341]

Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NB100-56341] - Analysis of HDAC-4 in Jurkat cell lysate with this antibody.

Applications for Histone Deacetylase 4/HDAC4 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation

1:20-1:1000. Use reported in scientific literature (Caretti et al (2003); Imbriano (2005))

Chromatin Immunoprecipitation (ChIP)

1:20-1:1000

Immunoprecipitation

assay dependent

Western Blot

0.5-2 ug/ml
Application Notes
A 140 kDa band is observed.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone Deacetylase 4/HDAC4

Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins. Eight members of HDAC family have been identified in the past several years. These HDAC family members are divided into two classes, I and II. Class I of the HDAC family comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about twofold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns. These results suggested that each member of the HDAC family exhibits a different, individual substrate specificity and function in vivo.

Long Name

Histone Deacetylase 4

Alternate Names

HDAC4

Entrez Gene IDs

9759 (Human); 208727 (Mouse)

Gene Symbol

HDAC4

UniProt

NP_848510 (Human)

Additional Histone Deacetylase 4/HDAC4 Products

Product Documents for Histone Deacetylase 4/HDAC4 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Histone Deacetylase 4/HDAC4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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