HIST1H3A Antibody (CL4967) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61150
![Western Blot: HIST1H3A Antibody (CL4967) [NBP2-61150]](https://resources.bio-techne.com/images/products/HIST1H3A-Antibody-CL4967-Western-Blot-NBP2-61150-img0006.jpg "Western Blot: HIST1H3A Antibody (CL4967) [NBP2-61150]")
![Western Blot: HIST1H3A Antibody (CL4967) [NBP2-61150]](https://resources.bio-techne.com/images/products/HIST1H3A-Antibody-CL4967-Western-Blot-NBP2-61150-img0007.jpg "Western Blot: HIST1H3A Antibody (CL4967) [NBP2-61150]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # CL4967
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody was developed using a synthetic peptide derived from P68431, with the exact immunogen sequence remaining proprietary.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for HIST1H3A Antibody (CL4967) - BSA Free
Western Blot: HIST1H3A Antibody (CL4967) [NBP2-61150]
Western Blot: HIST1H3A Antibody (CL4967) [NBP2-61150] - Western blot analysis of purified human recombinant histone H3, CenH3, H3.3, H2A, H2B and H4.![Immunohistochemistry-Paraffin: HIST1H3A Antibody (CL4967) [NBP2-61150]](https://resources.bio-techne.com/images/products/HIST1H3A-Antibody-CL4967-Immunohistochemistry-NBP2-61150-img0004.jpg "Immunohistochemistry-Paraffin: HIST1H3A Antibody (CL4967) [NBP2-61150]")
Immunohistochemistry-Paraffin: HIST1H3A Antibody (CL4967) [NBP2-61150]
Immunohistochemistry-Paraffin: HIST1H3A Antibody (CL4967) [NBP2-61150] - Immunohistochemical staining of human kidney shows strong nuclear positivity in renal tubules cells.![Western Blot: HIST1H3A Antibody (CL4967) [NBP2-61150]](https://resources.bio-techne.com/images/products/HIST1H3A-Antibody-CL4967-Western-Blot-NBP2-61150-img0005.jpg "Western Blot: HIST1H3A Antibody (CL4967) [NBP2-61150]")
Western Blot: HIST1H3A Antibody (CL4967) [NBP2-61150]
Western Blot: HIST1H3A Antibody (CL4967) [NBP2-61150] - Western blot analysis in human cell line HeLa.![Immunohistochemistry-Paraffin: HIST1H3A Antibody (CL4967) [NBP2-61150]](https://resources.bio-techne.com/images/products/HIST1H3A-Antibody-CL4967-Immunohistochemistry-NBP2-61150-img0001.jpg "Immunohistochemistry-Paraffin: HIST1H3A Antibody (CL4967) [NBP2-61150]")
![Immunohistochemistry-Paraffin: HIST1H3A Antibody (CL4967) [NBP2-61150]](https://resources.bio-techne.com/images/products/HIST1H3A-Antibody-CL4967-Immunohistochemistry-NBP2-61150-img0002.jpg "Immunohistochemistry-Paraffin: HIST1H3A Antibody (CL4967) [NBP2-61150]")
![Immunohistochemistry-Paraffin: HIST1H3A Antibody (CL4967) [NBP2-61150]](https://resources.bio-techne.com/images/products/HIST1H3A-Antibody-CL4967-Immunohistochemistry-NBP2-61150-img0003.jpg "Immunohistochemistry-Paraffin: HIST1H3A Antibody (CL4967) [NBP2-61150]")
![HIST1H3A Antibody (CL4967) - BSA Free Immunocytochemistry/ Immunofluorescence: HIST1H3A Antibody (CL4967) [NBP2-61150]](https://resources.bio-techne.com/images/products/nbp2-61150_-immunocytochemistry-immunofluorescence-639174077037187467.jpg "Immunocytochemistry/ Immunofluorescence: HIST1H3A Antibody (CL4967) [NBP2-61150]")
Applications for HIST1H3A Antibody (CL4967) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:10000 - 1:20000
Immunohistochemistry-Paraffin
1:10000 - 1:20000
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HIST1H3A
Alternate Names
H3 histone family, member A, H3/A, H3FAHIST1H3E, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, histone 1, H3a, histone cluster 1, H3a, histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l
Gene Symbol
H3C1
Additional HIST1H3A Products
Product Documents for HIST1H3A Antibody (CL4967) - BSA Free
Product Specific Notices for HIST1H3A Antibody (CL4967) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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