Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

CoraFluor 1

Antibody Source

Monoclonal Mouse IgG1 Clone # 927312

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Hexokinase 1/2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Hexokinase 2.
Phe11-Arg917
Accession # P52789

Specificity

Detects human Hexokinase 2 in direct ELISAs. Detects human Hexokinase 1 and human Hexokinase 2 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

CoraFluor(TM) 1, amine reactive

CoraFluor(TM) 1, thiol reactive

For more information, please see our CoraFluor(TM) TR-FRET technology flyer.

Scientific Data Images for Hexokinase 1/2 Antibody (927312) [CoraFluor™ 1]

Hexokinase 1/2 Antibody (927312) [CoraFluor™ 1]

Product Feature: CoraFluor Probes for TR-FRET

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

Applications for Hexokinase 1/2 Antibody (927312) [CoraFluor™ 1]

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Hexokinase 1/2

An alteration in the permeability of the mitochondrial membrane is a hallmark of the intrinsic pathway of caspase activation. This change in permeability leads to the cytosolic release of multiple proteins, including cytochrome c, Smac/Diablo, HTRA2/Omi, Apoptosis Inducing Factor (AIF), and Endonuclease G from the intermembrane space of the mitochondria. The release of these proteins, which are normally sequestered, has severe consequences for the cell. For example, cytoplasmic cytochrome c and dATP initiate the assembly of the apoptosome with APAF-1 and pro-caspase-9. The assembly of this complex promotes the activation of caspase-9 which subsequently leads to the activation of caspase-3, -6, and -7 to induce apoptosis. In addition, Smac/Diablo and HTRA2/Omi interact with the IAP proteins disrupting their abilities to inhibit caspase activity, while AIF and Endonuclease G translocate to the nucleus upon mitochondrial release and promote chromatin condensation and DNA fragmentation.

Additional Hexokinase 1/2 Products

Product Documents for Hexokinase 1/2 Antibody (927312) [CoraFluor™ 1]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Hexokinase 1/2 Antibody (927312) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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