HDAC10 Antibody (24GB6050)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50999

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NBP3-50999-100ul
NBP3-50999-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB6050

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HDAC10 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human HDAC10 (Uniprot: Q969S8)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HDAC10 Antibody (24GB6050)

HDAC10 Antibody (24GB6050)

Immunocytochemistry/ Immunofluorescence: HDAC10 Antibody (24GB6050)[NBP3-50999] -

Immunocytochemical staining of HepG2 cells with HDAC10 antibody (1:1,000). Nuclei were stained blue with DAPI; Histone deacetylase 10 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
HDAC10 Antibody (24GB6050)

Flow Cytometry: HDAC10 Antibody (24GB6050)[NBP3-50999] -

Flow cytometric analysis of HDAC10expression in HepG2 cells using HDAC10 antibody (1:2,000). Green, isotype control; red, histone deacetylase 10.
HDAC10 Antibody (24GB6050)

Western Blot: HDAC10 Antibody (24GB6050)[NBP3-50999] -

Western blotting analysis using HDAC10 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.
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Applications for HDAC10 Antibody (24GB6050)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HDAC10

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (for p300/CBP-associated factor), p300/CBP and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated mammalian RPD3) and HDAC3, all of which are related to the yeast transcriptional regulator Rpd3p, have been identified as histone deacetylases.

Long Name

Histone deacetylase 10

Alternate Names

EC 3.5.1.48, EC 3.5.1.62, HD10

Gene Symbol

HDAC10

Additional HDAC10 Products

Product Documents for HDAC10 Antibody (24GB6050)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HDAC10 Antibody (24GB6050)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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