Haptoglobin Antibody (7C1N2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16700
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 7C1N2 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 307-406 of human Haptoglobin (P00738). DQCIRHYEGSTVPEKKTPKSPVGVQPILNEHTFCAGMSKYQEDTCYGDAGSAFAVHDLEEDTWYATGILSFDKSCAVAEYGVYVKVTSIQDWVQKTIAEN
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit Haptoglobin Antibody (7C1N2) (NBP3-16700) is a recombinant monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Haptoglobin Antibody (7C1N2)
Western Blot: Haptoglobin Antibody (7C1N2) [NBP3-16700]
Western Blot: Haptoglobin Antibody (7C1N2) [NBP3-16700] - Western blot analysis of extracts of various cell lines, using Haptoglobin (HP) Rabbit mAb (NBP3-16700) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.Immunohistochemistry: Haptoglobin Antibody (7C1N2) [NBP3-16700]
Immunohistochemistry: Haptoglobin Antibody (7C1N2) [NBP3-16700] - Immunofluorescence analysis of rat liver using Haptoglobin (HP) Rabbit mAb (NBP3-16700) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunohistochemistry-Paraffin: Haptoglobin Antibody (7C1N2) [NBP3-16700]
Immunohistochemistry-Paraffin: Haptoglobin Antibody (7C1N2) [NBP3-16700] - Immunohistochemistry of paraffin-embedded human liver cancer using Haptoglobin (HP) Rabbit mAb (NBP3-16700) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Applications for Haptoglobin Antibody (7C1N2)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 ug/mL
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Haptoglobin
Alternate Names
HP, HP-1, hp2-alpha, HPA1S
Gene Symbol
HP
Additional Haptoglobin Products
Product Documents for Haptoglobin Antibody (7C1N2)
Product Specific Notices for Haptoglobin Antibody (7C1N2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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