GSK-3 beta Antibody (M131)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23366
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # M131
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Clone M131 was generated from a recombinant protein containing amino acid residues in the N-terminal region of human GSK-3 beta. This sequence is highly conserved in rat and mouse GSK-3 beta, and has 65% homology to similar regions in GSK-3 alpha. [UniProt# P49841]
Specificity
The antibody detects a 46 kDa protein corresponding to the apparent molecular mass of GSK-3 beta on SDS-PAGE immunoblots of rabbit fibroblasts, mouse brain, as well as human SKN-SH and A431 cells.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for GSK-3 beta Antibody (M131)
Western Blot: GSK-3 beta Antibody (M131) [NBP3-23366] -
Western Blot: GSK-3 beta Antibody (M131) [NBP3-23366] - Western blot analysis of a human recombinant GSK-3 beta N-terminal fragment (lanes 1 & 2) and endogenous GSK-3 beta expressed in mouse brain (lanes 3 & 4). The blot was probed with mouse monoclonal anti-GSK-3 beta at 1:500 (lanes 1 & 3) and 1:2000 (lanes 2 & 4).
Immunocytochemistry/Immunofluorescence: GSK-3 beta Antibody (M131) [NBP3-23366] -
Immunocytochemistry/Immunofluorescence: GSK-3 beta Antibody (M131) [NBP3-23366] - Immunocytochemical labeling of GSK-3 beta in aldehyde-fixed and NP-40 permeabilized human NCI-H1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-GSK-3 beta (NBP3-23366) antibody. The antibody was detected using appropriate secondary antibody conjugated to DyLight(R) 594.Applications for GSK-3 beta Antibody (M131)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50
Immunohistochemistry
1:250
Western Blot
1:500
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: GSK-3 beta
Long Name
Glycogen Synthase Kinase 3 beta
Alternate Names
GSK3 beta, GSK3B
Gene Symbol
GSK3B
Additional GSK-3 beta Products
Product Documents for GSK-3 beta Antibody (M131)
Product Specific Notices for GSK-3 beta Antibody (M131)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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