Granzyme B Antibody (CL10662) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44068
![Granzyme B Antibody (CL10662) - Azide and BSA Free Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP3-44068]](https://resources.bio-techne.com/images/products/nbp3-44068_mouse-granzyme-b-mab-cl10662-azide-and-bsa-free-282202513112820.jpg "Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP3-44068]")
![Granzyme B Antibody (CL10662) - Azide and BSA Free Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP3-44068]](https://resources.bio-techne.com/images/products/nbp3-44068_mouse-granzyme-b-mab-cl10662-azide-and-bsa-free-282202513381581.jpg "Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP3-44068]")
![Granzyme B Antibody (CL10662) - Azide and BSA Free Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP3-44068]](https://resources.bio-techne.com/images/products/nbp3-44068_mouse-granzyme-b-mab-cl10662-azide-and-bsa-free-28220251215417.jpg "Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP3-44068]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL10662
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
This antibody was generated using a recombinant protein sequence of P10144, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Granzyme B Antibody (CL10662) - Azide and BSA Free
![Granzyme B Antibody (CL10662) - Azide and BSA Free Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP3-44068]](https://resources.bio-techne.com/images/products/nbp3-44068_mouse-granzyme-b-mab-cl10662-azide-and-bsa-free-282202512332999.jpg "Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP3-44068]")
Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP3-44068]
Staining of human cerebral cortex shows no positivity in neurons as expected.![Granzyme B Antibody (CL10662) - Azide and BSA Free Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP3-44068]](https://resources.bio-techne.com/images/products/nbp3-44068_mouse-granzyme-b-mab-cl10662-azide-and-bsa-free-28220251331455.jpg "Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP3-44068]")
Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP3-44068]
Staining of human rectum shows strong cytoplasmic positivity in a subset of lymphoid cells.Applications for Granzyme B Antibody (CL10662) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Granzyme B
Granzyme family serine proteases are stored in the granules of cytotoxic T lymphocytes and natural killer cells. Granzymes contain one S1 protease domain and are synthesized as preproproteins. Granzymes are released toward pathogen infected or transformed target cells during cellular immune reactions. Target cell entry by granzymes is through perforin channels. Granzymes trigger apoptosis by caspase-dependent and -independent mechanisms. There are five granzymes (A, B, G, H and K) in human and many more in mouse and rat.
Alternate Names
CGL-1, CGL1, CSPB, CTLA-1, CTLA1, CTSGL1, Fragmentin-2, Granzyme-2, GRB, GrzB, GZMB, HLP, SECT
Gene Symbol
GZMB
Additional Granzyme B Products
Product Documents for Granzyme B Antibody (CL10662) - Azide and BSA Free
Product Specific Notices for Granzyme B Antibody (CL10662) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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