Granzyme B Antibody (B18.1) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97525
![Flow Cytometry: Granzyme B Antibody (B18.1) - BSA Free [NBP1-97525]](https://resources.bio-techne.com/images/products/Granzyme-B-Antibody-B18-1-Flow-Cytometry-NBP1-97525-img0002.jpg "Flow Cytometry: Granzyme B Antibody (B18.1) - BSA Free [NBP1-97525]")
![Flow Cytometry: Granzyme B Antibody (B18.1) - BSA Free [NBP1-97525]](https://resources.bio-techne.com/images/products/Granzyme-B-Antibody-B18-1-Flow-Cytometry-NBP1-97525-img0006.jpg "Flow Cytometry: Granzyme B Antibody (B18.1) - BSA Free [NBP1-97525]")
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Cited:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # B18.1
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Recombinant human granzyme B (aa 7-227).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Granzyme B Antibody (B18.1) - BSA Free
Flow Cytometry: Granzyme B Antibody (B18.1) - BSA Free [NBP1-97525]
Flow Cytometry: Granzyme B Antibody (B18.1) [NBP1-97525] - An intracellular stain was performed on U937 cells with Granzyme B [B18.1] Antibody NBP1-97525AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.![Western Blot: Granzyme B Antibody (B18.1)BSA Free [NBP1-97525]](https://resources.bio-techne.com/images/products/Granzyme-B-Antibody-B18-1-Western-Blot-NBP1-97525-img0001.jpg "Western Blot: Granzyme B Antibody (B18.1)BSA Free [NBP1-97525]")
Western Blot: Granzyme B Antibody (B18.1)BSA Free [NBP1-97525]
Western Blot: Granzyme B Antibody (B18.1) [NBP1-97525] - Detection of recombinant granzyme B. Granzyme B migrates as a 27 kDa species. Method: Recombinant granzyme B was resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-granzyme B antibody B18.1 at 1ug/ml. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG (SBA) and a chemiluminescence detection system![Immunocytochemistry/ Immunofluorescence: Granzyme B Antibody (B18.1) - BSA Free [NBP1-97525]](https://resources.bio-techne.com/images/products/Granzyme-B-Antibody-B18-1-Immunocytochemistry-Immunofluorescence-NBP1-97525-img0005.jpg "Immunocytochemistry/ Immunofluorescence: Granzyme B Antibody (B18.1) - BSA Free [NBP1-97525]")
Immunocytochemistry/ Immunofluorescence: Granzyme B Antibody (B18.1) - BSA Free [NBP1-97525]
Immunocytochemistry/Immunofluorescence: Granzyme B Antibody (B18.1) [NBP1-97525] - Human T cells were plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20oC for 5 min, then in acetone at -20oC for 30 sec. After 3 washes in PBS, 0.1% BSA, slides were incubated for 1 hr at room temperature (RT) with 20 ug/ml of B18.4 antibody in PBS, 0.1% BSA. After rinsing in PBS, a FITC-conjugated anti-mouse IgG was added for 30 min., slides washed again in PBS and visualized using a fluorescence microscope.Applications for Granzyme B Antibody (B18.1) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
1:10-1:500
Western Blot
1:100-1:2000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Granzyme B
Granzyme family serine proteases are stored in the granules of cytotoxic T lymphocytes and natural killer cells. Granzymes contain one S1 protease domain and are synthesized as preproproteins. Granzymes are released toward pathogen infected or transformed target cells during cellular immune reactions. Target cell entry by granzymes is through perforin channels. Granzymes trigger apoptosis by caspase-dependent and -independent mechanisms. There are five granzymes (A, B, G, H and K) in human and many more in mouse and rat.
Alternate Names
CGL-1, CGL1, CSPB, CTLA-1, CTLA1, CTSGL1, Fragmentin-2, Granzyme-2, GRB, GrzB, GZMB, HLP, SECT
Gene Symbol
GZMB
Additional Granzyme B Products
Product Documents for Granzyme B Antibody (B18.1) - BSA Free
Product Specific Notices for Granzyme B Antibody (B18.1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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