Grancalcin Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89786

Novus Biologicals, part of Bio-Techne
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NBP1-89786
NBP1-89786-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ALNAWKENFMTVDQDGSGTVEHHELRQAIGLMGYRLSPQTLTTIVKRYSKNGRIFFDDY

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (85%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Grancalcin Antibody - BSA Free (NBP1-89786) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Grancalcin Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Grancalcin Antibody [NBP1-89786]

Immunocytochemistry/ Immunofluorescence: Grancalcin Antibody [NBP1-89786]

Immunocytochemistry/Immunofluorescence: Grancalcin Antibody [NBP1-89786] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Immunohistochemistry-Paraffin: Grancalcin Antibody [NBP1-89786]

Immunohistochemistry-Paraffin: Grancalcin Antibody [NBP1-89786]

Immunohistochemistry-Paraffin: Grancalcin Antibody [NBP1-89786] - Staining of human bone marrow, cerebral cortex, kidney and liver using Anti-GCA antibody NBP1-89786 (A) shows similar protein distribution across tissues to independent antibody NBP1-89785 (B).
Grancalcin Antibody - BSA Free Immunohistochemistry-Paraffin: Grancalcin Antibody - BSA Free [NBP1-89786]

Immunohistochemistry-Paraffin: Grancalcin Antibody - BSA Free [NBP1-89786]

Analysis in human bone marrow and cerebral cortex tissues using Anti-GCA antibody. Corresponding GCA RNA-seq data are presented for the same tissues.
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Applications for Grancalcin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Grancalcin

Grancalcin, is a calcium-binding protein abundant in neutrophils and macrophages. It belongs to the penta-EF-hand subfamily of proteins which includes sorcin, calpain, and ALG-2. Grancalcin localization is dependent upon calcium and magnesium. In the absence of divalent cation, grancalcin localizes to the cytosolic fraction; with magnesium alone, it partitions with the granule fraction; and in the presence of magnesium and calcium, it associates with both the granule and membrane fractions, suggesting a role for grancalcin in granule-membrane fusion and degranulation.

Alternate Names

EF-hand calcium-binding protein, grancalcin, EF-hand calcium binding protein, grancalcin, penta-EF-hand protein

Gene Symbol

GCA

Additional Grancalcin Products

Product Documents for Grancalcin Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Grancalcin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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