Grancalcin Antibody (2F5) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # H00025801-M01
Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 2F5
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
GCA (AAH05214, 1 a.a. ~ 217 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAYPGYGGGFGNFSIQVPGMQMGQPVPETGPAILLDGYSGPAYSDTYSSAGDSVYTYFSAVAGQDGEVDAEELQRCLTQSGINGTYSPFSLETCRIMIAMLDRDHTGKMGFNAFKELWAALNAWKENFMTVDQDGSGTVEHHELRQAIGLMGYRLSPQTLTTIVKRYSKNGRIFFDDYVACCVKLRALTDFFRKRDHLQQGSANFIYDDFLQGTMAI
Reactivity Notes
Human. Other species not tested.
Specificity
GCA (2F5)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Novus Biologicals Mouse Grancalcin Antibody (2F5) - Azide and BSA Free (H00025801-M01) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-Grancalcin Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Grancalcin Antibody (2F5) - Azide and BSA Free
![Western Blot: Grancalcin Antibody (2F5) [H00025801-M01]](https://resources.bio-techne.com/images/products/Grancalcin-Antibody-2F5-Western-Blot-H00025801-M01-img0006.jpg "Western Blot: Grancalcin Antibody (2F5) [H00025801-M01]")
Western Blot: Grancalcin Antibody (2F5) [H00025801-M01]
Western Blot: Grancalcin Antibody (2F5) [H00025801-M01] - GCA monoclonal antibody (M01), clone 2F5 Analysis of GCA expression in MCF-7.![Immunocytochemistry/ Immunofluorescence: Grancalcin Antibody (2F5) [H00025801-M01]](https://resources.bio-techne.com/images/products/Grancalcin-Antibody-2F5-Immunocytochemistry-Immunofluorescence-H00025801-M01-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Grancalcin Antibody (2F5) [H00025801-M01]")
Immunocytochemistry/ Immunofluorescence: Grancalcin Antibody (2F5) [H00025801-M01]
Immunocytochemistry/Immunofluorescence: Grancalcin Antibody (2F5) [H00025801-M01] - Analysis of monoclonal antibody to GCA on MCF-7 cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: Grancalcin Antibody (2F5) [H00025801-M01] - Detection limit for recombinant GST tagged GCA is 0.03 ng/ml as a capture antibody.
Applications for Grancalcin Antibody (2F5) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Grancalcin
Alternate Names
EF-hand calcium-binding protein, grancalcin, EF-hand calcium binding protein, grancalcin, penta-EF-hand protein
Entrez Gene IDs
25801 (Human)
Gene Symbol
GCA
OMIM
607030 (Human)
UniProt
Additional Grancalcin Products
Product Documents for Grancalcin Antibody (2F5) - Azide and BSA Free
Product Specific Notices for Grancalcin Antibody (2F5) - Azide and BSA Free
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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