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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Summary for GPR35 Antibody

Immunogen

A portion of amino acids 275-309 of human G Protein-Coupled Receptor GPR35 was used as the immunogen.

Reactivity Notes

Human reactivity reported in scientific literature (PMID:32755573).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GPR35 Antibody

Western Blot: GPR35 Antibody [NBP2-24640] - Analysis of G Protein-Coupled Receptor GPR35 in MCF7 cell lysate in the 1) absence and 2) presence of immunizing peptide 3) RAW using NBP2-24640 at 0.5 ug/ml and 1.0 ug/ml, respectively.
Immunohistochemistry-Paraffin: GPR35 Antibody [NBP2-24640] - Analysis of GPR35 in paraffin-embedded formalin-fixed MCF7cells using an isotype control (top left) and NBP2-24640 (bottom left, right) at 5 ug/ml.

Applications for GPR35 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

5 ug/ml

Western Blot

0.5-1.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 8 publications using NBP2-24640 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GPR35

GPR35 is an Orphan-A GPCR with an unknown ligand. GPR35 expression was reported only in rat intestine by Northern hybridization, however, another study showed that GPR35 is expressed widely, including pancreatic islets and skeletal muscle, with higher levels in adult lung, small intestine, colon, and stomach. ESTs have been isolated from human fetal lung/testis/B-cell, colon (normal, cancer, and Crohn's disease), breast cancer, pancreatic cancer, and leukemia libraries.

Long Name

G Protein-coupled Receptor 35

Alternate Names

G protein-coupled receptor 35, G-protein coupled receptor 35, KYNA receptor, Kynurenic acid receptor

Gene Symbol

GPR35

Product Documents for GPR35 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GPR35 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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