GPR3 Antibody (3B4-G3) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # H00002827-M01
![Western Blot: GPR3 Antibody (3B4-G3) [H00002827-M01]](https://resources.bio-techne.com/images/products/GPR3-Antibody-3B4-G3-Western-Blot-H00002827-M01-img0010.jpg "Western Blot: GPR3 Antibody (3B4-G3) [H00002827-M01]")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 3B4-G3
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
GPR3 (AAH32702, 1 a.a. ~ 330 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MMWGAGSPLAWLSAGSGNVNVSSVGPAEGPTGPAAPLPSPKAWDVVLCISGTLVSCENALVVAIIVGTPAFRAPMFLLVGSLAVADLLAGLGLVLHFAAVFCIGSAEMSLVLVGVLAMAFTASIGSLLAITVDRYLSLYNALTYYSETTVTRTYVMLALVWGGALGLGLLPVLAWNCLDGLTTCGVVYPLSKNHLVVLAIAFFMVFGIMLQLYAQICRIVCRHAQQIALQRHLLPASHYVATRKGIATLAVVLGAFAACWLPFTVYCLLGDAHSPPLYTYLTLLPATYNSMINPIIYAFRNQDVQKVLWAVCCCCSSSKIPFRSRSPSDV
Specificity
GPR3 - G protein-coupled receptor 3
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for GPR3 Antibody (3B4-G3) - Azide and BSA Free
![Western Blot: GPR3 Antibody (3B4-G3) [H00002827-M01]](https://resources.bio-techne.com/images/products/GPR3-Antibody-3B4-G3-Western-Blot-H00002827-M01-img0011.jpg "Western Blot: GPR3 Antibody (3B4-G3) [H00002827-M01]")
Western Blot: GPR3 Antibody (3B4-G3) [H00002827-M01]
Western Blot: GPR3 Antibody (3B4-G3) [H00002827-M01] - Analysis of GPR3 expression in transfected 293T cell line by GPR3 monoclonal antibody (M01), clone 3B4-G3.Lane 1: GPR3 transfected lysate(35 KDa).Lane 2: Non-transfected lysate.![Immunohistochemistry-Paraffin: GPR3 Antibody (3B4-G3) [H00002827-M01]](https://resources.bio-techne.com/images/products/GPR3-Antibody-3B4-G3-Immunohistochemistry-Paraffin-H00002827-M01-img0007.jpg "Immunohistochemistry-Paraffin: GPR3 Antibody (3B4-G3) [H00002827-M01]")
Immunohistochemistry-Paraffin: GPR3 Antibody (3B4-G3) [H00002827-M01]
Immunohistochemistry-Paraffin: GPR3 Antibody (3B4-G3) [H00002827-M01] - Analysis of monoclonal antibody to GPR3 on formalin-fixed paraffin-embedded human transitional cell carcinoma tissue. Antibody concentration 1 ug/ml.![Western Blot: GPR3 Antibody (3B4-G3) [H00002827-M01]](https://resources.bio-techne.com/images/products/GPR3-Antibody-3B4-G3-Western-Blot-H00002827-M01-img0008.jpg "Western Blot: GPR3 Antibody (3B4-G3) [H00002827-M01]")
Western Blot: GPR3 Antibody (3B4-G3) [H00002827-M01]
Western Blot: GPR3 Antibody (3B4-G3) [H00002827-M01] - Analysis of GPR3 over-expressed 293 cell line, cotransfected with GPR3 Validated Chimera RNAi ( Cat # H00002827-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with GPR3 monoclonal antibody (M01), clone 3B4-G3 (Cat # H00002827-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Applications for GPR3 Antibody (3B4-G3) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P, RNAi Validation and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: GPR3
GPR3, previously called ACCA (adenylate cyclase constitutive activator), is 7-transmembrane receptor that has been shown to promote cAMP accumulation. In oocytes, cAMP generated by GPR3 regulates maintenance of meiotic arrest. GPR3 is also expressed in the brain, where it has been implicated in cAMP-dependent emotional responses and modulation of amyloid-beta peptide generation.
Long Name
G Protein-coupled Receptor 3
Alternate Names
ACCA, G protein-coupled receptor 3
Entrez Gene IDs
2827 (Human)
Gene Symbol
GPR3
OMIM
600241 (Human)
UniProt
Additional GPR3 Products
Product Documents for GPR3 Antibody (3B4-G3) - Azide and BSA Free
Product Specific Notices for GPR3 Antibody (3B4-G3) - Azide and BSA Free
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...