GPR18 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24918
![Flow Cytometry: GPR18 Antibody - BSA Free [NBP2-24918]](https://resources.bio-techne.com/images/products/GPR18-Antibody-Flow-Cytometry-NBP2-24918-img0007.jpg "Flow Cytometry: GPR18 Antibody - BSA Free [NBP2-24918]")
![Western Blot: GPR18 AntibodyBSA Free [NBP2-24918]](https://resources.bio-techne.com/images/products/GPR18-Antibody-Western-Blot-NBP2-24918-img0001.jpg "Western Blot: GPR18 AntibodyBSA Free [NBP2-24918]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Cited:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Partial synthetic peptide made to an N-terminal portion of the human GPR18 protein (between amino acids 1-50) [UniProt Q14330]
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for GPR18 Antibody - BSA Free
Western Blot: GPR18 AntibodyBSA Free [NBP2-24918]
Western Blot: GPR18 Antibody [NBP2-24918] - Analysis of human spleen lysate (4 ug/mL) in the 1) absence and 2) presence of immunizing peptide 3) mouse spleen (6 ug/mL) and 4) rat spleen lysate (3 ug/mL) using GPR18 antibody.![Immunocytochemistry/ Immunofluorescence: GPR18 Antibody - BSA Free [NBP2-24918]](https://resources.bio-techne.com/images/products/GPR18-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24918-img0006.jpg "Immunocytochemistry/ Immunofluorescence: GPR18 Antibody - BSA Free [NBP2-24918]")
Immunocytochemistry/ Immunofluorescence: GPR18 Antibody - BSA Free [NBP2-24918]
Immunocytochemistry/Immunofluorescence: GPR18 Antibody [NBP2-24918] - Ntera2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-GPR18 Antibody NBP2-24918 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.![Immunohistochemistry-Paraffin: GPR18 Antibody - BSA Free [NBP2-24918]](https://resources.bio-techne.com/images/products/GPR18-Antibody-Immunohistochemistry-Paraffin-NBP2-24918-img0003.jpg "Immunohistochemistry-Paraffin: GPR18 Antibody - BSA Free [NBP2-24918]")
Immunohistochemistry-Paraffin: GPR18 Antibody - BSA Free [NBP2-24918]
Immunohistochemistry-Paraffin: GPR18 Antibody [NBP2-24918] - Analysis of a FFPE tissue section of human tonsil using 1:200 dilution of GPR18 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.![Immunohistochemistry-Paraffin: GPR18 Antibody - BSA Free [NBP2-24918]](https://resources.bio-techne.com/images/products/GPR18-Antibody-Immunohistochemistry-Paraffin-NBP2-24918-img0002.jpg "Immunohistochemistry-Paraffin: GPR18 Antibody - BSA Free [NBP2-24918]")
![Flow Cytometry: GPR18 Antibody - BSA Free [NBP2-24918]](https://resources.bio-techne.com/images/products/GPR18-Antibody-Flow-Cytometry-NBP2-24918-img0004.jpg "Flow Cytometry: GPR18 Antibody - BSA Free [NBP2-24918]")
![Flow Cytometry: GPR18 Antibody - BSA Free [NBP2-24918]](https://resources.bio-techne.com/images/products/GPR18-Antibody-Flow-Cytometry-NBP2-24918-img0005.jpg "Flow Cytometry: GPR18 Antibody - BSA Free [NBP2-24918]")
Applications for GPR18 Antibody - BSA Free
Application
Recommended Usage
Flow Cytometry
reported in scientific literature (PMID 27256622)
Immunocytochemistry/ Immunofluorescence
2 - 10 ug/ml. Use reported in scientific literature (PMID 30894369)
Immunohistochemistry
1:200
Immunohistochemistry-Paraffin
1:200
Western Blot
2 ug/ml
Reviewed Applications
Read 1 review rated 3 using NBP2-24918 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: GPR18
Long Name
G Protein-coupled Receptor 18
Alternate Names
GPCRW, NAGly Receptor
Gene Symbol
GPR18
Additional GPR18 Products
Product Documents for GPR18 Antibody - BSA Free
Product Specific Notices for GPR18 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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