GPER/GPR30 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31258
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GPER/GPR30. The exact sequence is proprietary.
Localization
Cell membrane, Multi-pass membrane protein, Endoplasmic reticulum membrane, Multi-pass membrane protein, Golgi apparatus membrane, Multi-pass membrane protein
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit GPER/GPR30 Antibody - BSA Free (NBP1-31258) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for GPER/GPR30 Antibody - BSA Free
Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NBP1-31258]
Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NBP1-31258] - Paraffin-embedded endometrial, using antibody at 1:150 dilution.Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NBP1-31258]
Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NBP1-31258] - Paraffin-embedded endometrial, using antibody at 1:150 dilution.Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NBP1-31258] -
Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NBP1-31258] - GPER/GPR30 antibody [C2C3], C-term detects GPER/GPR30 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human colon cancer.GPER/GPR30 stained by GPER/GPR30 antibody [C2C3], C-term (NBP1-31258) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 minApplications for GPER/GPR30 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Frozen
Assay dependent
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Application Notes
WB-As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: GPER/GPR30
Long Name
G Protein-coupled Estrogen Receptor 1
Alternate Names
CEPR, CMKRL2, DRY12, FEG-1, GPCR-BR, GPR30, LERGU2, LyGPR
Entrez Gene IDs
2852 (Human)
Gene Symbol
GPER1
UniProt
Additional GPER/GPR30 Products
Product Documents for GPER/GPR30 Antibody - BSA Free
Product Specific Notices for GPER/GPR30 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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