Golgi Complex Antibody (SPM581) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34813
![Immunohistochemistry-Paraffin: Golgi Complex Antibody (SPM581) - Azide and BSA Free [NBP2-34813] Immunohistochemistry-Paraffin: Golgi Complex Antibody (SPM581) - Azide and BSA Free [NBP2-34813]](https://resources.bio-techne.com/images/products/Golgi-Complex-Antibody-SPM581-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34813-img0001.jpg)
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM581
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
SU-DHL-1 large cell lymphoma cells.
Localization
Golgi complex in cytoplasm
Specificity
This monoclonal antibody recognizes Golgi complex in human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells and may be used to stain Golgi complex of cells in frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. This monoclonal antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells.
Marker
Marker for Human Cells
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA. Also available at 200 ug/ml WITH BSA & azide (NBP2-32853).
Antibody with azide - store at 2 to 8C.
Antibody with azide - store at 2 to 8C.
Scientific Data Images for Golgi Complex Antibody (SPM581) - Azide and BSA Free
Immunohistochemistry-Paraffin: Golgi Complex Antibody (SPM581) - Azide and BSA Free [NBP2-34813]
Immunohistochemistry-Paraffin: Golgi Complex Antibody (SPM581) - Azide and BSA Free [NBP2-34813] - Formalin-fixed, paraffin-embedded human Gallbladder stained with Golgi Monoclonal Antibody (SPM581).Applications for Golgi Complex Antibody (SPM581) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.5-1ug/ml
Application Notes
Immunocytochemistry (Acetone or paraformaldehyde fixed): 1-2ug/ml for 30 min.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Golgi Complex
Alternate Names
AI593353;AW537898;CFR;CFR1;CFR-1;ESL1;ESL-1;GLG1;MG160;MG-160;Selel
Additional Golgi Complex Products
Product Documents for Golgi Complex Antibody (SPM581) - Azide and BSA Free
Product Specific Notices for Golgi Complex Antibody (SPM581) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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