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Golgi Complex Antibody (AE-6) - IHC-Prediluted

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48040

Novus Biologicals, part of Bio-Techne
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NBP2-48040

Key Product Details

Species Reactivity

Human, Mouse (Negative), Rat (Negative)

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # AE-6

Format

IHC-Prediluted

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

SU-DHL-1 large cell lymphoma cells

Reactivity Notes

Does not react with Mouse or Rat.

Localization

Golgi complex in cytoplasm

Specificity

This monoclonal antibody recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This monoclonal antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae.

Marker

Marker for Human Cells

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for Golgi Complex Antibody (AE-6) - IHC-Prediluted

Immunohistochemistry-Paraffin: Golgi Complex Antibody (AE-6) - IHC-Prediluted [NBP2-48040]

Immunohistochemistry-Paraffin: Golgi Complex Antibody (AE-6) - IHC-Prediluted [NBP2-48040]

Immunohistochemistry-Paraffin: Golgi Complex Antibody (AE-6) - IHC-Prediluted [NBP2-48040] - Human Pancreas stained with Golgi Monoclonal Antibody (AE-6).

Applications for Golgi Complex Antibody (AE-6) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunocytochemistry Acetone or paraformaldehyde fixed 0.5 - 1 ug/ml for 30 min; Immunohistochemistry-Paraffin 0.5 - 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Golgi Complex

Alternate Names

AI593353;AW537898;CFR;CFR1;CFR-1;ESL1;ESL-1;GLG1;MG160;MG-160;Selel

Additional Golgi Complex Products

Product Documents for Golgi Complex Antibody (AE-6) - IHC-Prediluted

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Golgi Complex Antibody (AE-6) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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