Golgi Complex Antibody (AE-6) - IHC-Prediluted
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48040
![Immunohistochemistry-Paraffin: Golgi Complex Antibody (AE-6) - IHC-Prediluted [NBP2-48040] Immunohistochemistry-Paraffin: Golgi Complex Antibody (AE-6) - IHC-Prediluted [NBP2-48040]](https://resources.bio-techne.com/images/products/Golgi-Complex-Antibody-AE-6-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48040-img0001.jpg)
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse (Negative), Rat (Negative)
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # AE-6
Format
IHC-Prediluted
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
SU-DHL-1 large cell lymphoma cells
Reactivity Notes
Does not react with Mouse or Rat.
Localization
Golgi complex in cytoplasm
Specificity
This monoclonal antibody recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This monoclonal antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae.
Marker
Marker for Human Cells
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
Scientific Data Images for Golgi Complex Antibody (AE-6) - IHC-Prediluted
Immunohistochemistry-Paraffin: Golgi Complex Antibody (AE-6) - IHC-Prediluted [NBP2-48040]
Immunohistochemistry-Paraffin: Golgi Complex Antibody (AE-6) - IHC-Prediluted [NBP2-48040] - Human Pancreas stained with Golgi Monoclonal Antibody (AE-6).Applications for Golgi Complex Antibody (AE-6) - IHC-Prediluted
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunocytochemistry Acetone or paraformaldehyde fixed 0.5 - 1 ug/ml for 30 min; Immunohistochemistry-Paraffin 0.5 - 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Format
IHC-Prediluted
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Golgi Complex
Alternate Names
AI593353;AW537898;CFR;CFR1;CFR-1;ESL1;ESL-1;GLG1;MG160;MG-160;Selel
Additional Golgi Complex Products
Product Documents for Golgi Complex Antibody (AE-6) - IHC-Prediluted
Product Specific Notices for Golgi Complex Antibody (AE-6) - IHC-Prediluted
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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