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GM130/GOLGA2 Antibody (23GB5570)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51354

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NBP3-51354-100ul
NBP3-51354-20ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB5570

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthesized peptide derived from human GM130/GOLGA2 (Uniprot: Q08379)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GM130/GOLGA2 Antibody (23GB5570)

GM130/GOLGA2 Antibody (23GB5570)

Immunocytochemistry/ Immunofluorescence: GM130/GOLGA2 Antibody (23GB5570)[NBP3-51354] -

Immunocytochemical staining of HepG2 cells with GM130/GOLGA2 antibody (1:1,000). Nuclei were stained blue with DAPI; GM130/GOLGA2 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 um.
GM130/GOLGA2 Antibody (23GB5570)

Flow Cytometry: GM130/GOLGA2 Antibody (23GB5570)[NBP3-51354] -

Flow cytometric analysis of GM130/GOLGA2expression in HepG2 cells using GM130/GOLGA2 antibody (1:2,000). Green, isotype control; red, Golgin A2.
GM130/GOLGA2 Antibody (23GB5570)

Western Blot: GM130/GOLGA2 Antibody (23GB5570)[NBP3-51354] -

Western blotting analysis using GM130/GOLGA2 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.

Applications for GM130/GOLGA2 Antibody (23GB5570)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GM130/GOLGA2

Golgi Matrix Protein (GM130) is peripheral cytoplamic protein that is bound to the Golgi membranes. It has been implicated as a maintainer of cis-Golgi structure (1). During mitosis, it regulates the disassembly and reassembly of the Golgi complex. It is also linked to docking and fusion of coatomer (COP I) coated vesicles to the Golgi membrane (2). The COOH-terminal domain of GM130 is highly homologous to Golgi human auto-antigen, golgin-95. GM130 interacts specifically and GTP-dependent manner with Rab1b protein, a regulator of anterograde traffic between ER and Golgi membranes (3). GM130 is also activator of YSK1, an essential player in cell migration.

Long Name

Golgin A2

Alternate Names

GOLGA2, Golgin-95, SY11 Protein

Gene Symbol

GOLGA2

Additional GM130/GOLGA2 Products

Product Documents for GM130/GOLGA2 Antibody (23GB5570)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for GM130/GOLGA2 Antibody (23GB5570)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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