Glypican 3 Antibody (CL12711) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44385

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL12711

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Glypican 3 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of P51654, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Glypican 3 Antibody (CL12711) - Azide and BSA Free

Glypican 3 Antibody (CL12711) - Azide and BSA Free Immunohistochemistry-Paraffin: Glypican 3 Antibody [NBP3-44385]

Immunohistochemistry-Paraffin: Glypican 3 Antibody [NBP3-44385]

Analysis in human placenta and skeletal muscle tissues using NBP3-44385 antibody. Corresponding Glypican 3 RNA-seq data are presented for the same tissues.
Glypican 3 Antibody (CL12711) - Azide and BSA Free Immunohistochemistry-Paraffin: Glypican 3 Antibody [NBP3-44385]

Immunohistochemistry-Paraffin: Glypican 3 Antibody [NBP3-44385]

Staining of human kidney shows moderate membranous positivity in cells in tubules.
Glypican 3 Antibody (CL12711) - Azide and BSA Free Immunohistochemistry-Paraffin: Glypican 3 Antibody [NBP3-44385]

Immunohistochemistry-Paraffin: Glypican 3 Antibody [NBP3-44385]

Staining of human liver cancer (hepatocellular carcinoma) shows moderate membranous positivity in tumor cells.
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Applications for Glypican 3 Antibody (CL12711) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000 - 1:2500
Application Notes
For use with IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Glypican 3

The Glypican 3 (GPC3) gene encodes for a member of the glypican-related integral membrane proteoglycan family (GRIPS). Two isoforms of the protein exist, isoform 1 at a length of 580 amino acids long and 65 kDA while isoform 2 is 526 amino acids long at 59 kDA. The protein encoded by GPC3 is thought to prevent the dipeptidyl peptidase activity of DPP4 and may participate in the suppression and regulation of growth in the predominately mesodermal tissues and organs. The GPC3 gene participates in the Wnt signaling pathway, metabolism, and visual phototransduction through interactions with genes FGF2, WNT3A, WNT7B, BCAN, and BGN. GPC3 has been researched in various diseases such as paine syndrome, horner's sundrome, liver cirrhosis, simpson-golabi-behmel syndrome, germ cell tumors, wilms tumors, complex regional pain syndrome, and hepatocellulcar carcinoma.

Alternate Names

GPC3

Gene Symbol

GPC3

Additional Glypican 3 Products

Product Documents for Glypican 3 Antibody (CL12711) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Glypican 3 Antibody (CL12711) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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