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Glutamine Synthetase Antibody (CL3004) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43806

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL3004

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P15104, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Glutamine Synthetase Antibody (CL3004) - Azide and BSA Free

Glutamine Synthetase Antibody (CL3004) - Azide and BSA Free Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP3-43806]

Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP3-43806]

Staining of human cerebellum shows positivity in glial cells.
Glutamine Synthetase Antibody (CL3004) - Azide and BSA Free Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP3-43806]

Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP3-43806]

Staining of human kidney shows absence of immunoreactivity (negative control).
Glutamine Synthetase Antibody (CL3004) - Azide and BSA Free Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP3-43806]

Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP3-43806]

Staining of human cerebral cortex shows strong immunoreactivity in astrocytes.

Applications for Glutamine Synthetase Antibody (CL3004) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Glutamine Synthetase

Glutamine synthetase is part of the glutamine synthetase family. Specifically, Glutamine synthetase catalyzes the condensation of glutamate and ammonia to form glutamine, during the metabolism of nitrogen. Ammonia incorporation in animals occurs through the actions of glutamate dehydrogenase and glutamine synthase. Glutamate plays the central role in mammalian nitrogen flow, serving as both a nitrogen donor and nitrogen acceptor.

Glutamine synthetase antibodies are useful tools for studying nitrogen metabolism and regulation.

Alternate Names

cell proliferation-inducing protein 59, EC 4.1.1.15, EC 6.3.1.2, GLNSPIG59, Glutamate decarboxylase, glutamate-ammonia ligase, Glutamate--ammonia ligase, glutamate-ammonia ligase (glutamine synthase), glutamine synthase, glutamine synthetase, GS, PIG43, proliferation-inducing protein 43

Gene Symbol

GLUL

Additional Glutamine Synthetase Products

Product Documents for Glutamine Synthetase Antibody (CL3004) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Glutamine Synthetase Antibody (CL3004) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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