Key Product Details

Species Reactivity

Rat

Applications

Immunoaffinity Purification, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Janelia Fluor 669

Antibody Source

Monoclonal Mouse IgG1 Clone # 1F8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Glut4 Antibodies »

Product Specifications

Immunogen

Partially purified vesicles containing Glut4

Specificity

Recognizes an epitope in the cytoplasmic portion of rat Glut4 and has been shown to bind only to the muscle-adipose isoform of the glucose transporter, which is antigenically unique from the other transport proteins (1-3). Recognizes Glut4 in human, monkey, rat, mouse, and rabbit systems. It does not recognize this protein in canine systems. Its ability to bind to glucose transporter from other species has not been tested.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Glut4 Antibody (1F8) [Janelia Fluor® 669]

Application
Recommended Usage

Immunoaffinity Purification

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Glut4

The stimulation of glucose uptake by insulin in muscle and adipose tissue requires translocation of the GLUT4 glucose transporter from intracellular storage sites to the cell surface. Activation of phosphatidylinositol-3-OH kinase (PI3K) is required for this trafficking event, but it is not sufficient to produce GLUT4 translocation. Human insulin resistance involves a defect in GLUT4 traffic and targeting leading to accumulation in a dense membrane compartment from which insulin is unable to recruit GLUT4 to the cell surface.

Alternate Names

SLC2A4

Gene Symbol

SLC2A4

Additional Glut4 Products

Product Documents for Glut4 Antibody (1F8) [Janelia Fluor® 669]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Glut4 Antibody (1F8) [Janelia Fluor® 669]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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