Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-236
![Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236] Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236]](https://resources.bio-techne.com/images/products/Glucose-6-Phosphate-Dehydrogenase-Antibody-Western-Blot-NB100-236-img0009.jpg)
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunoblotting, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot
Cited:
Immunoblotting, Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residues 50 and 100 of human Glucose-6-Phosphate Dehydrogenase using the numbering given in entry NP_000393.2 (GeneID 2539).
Marker
Cytosol Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free (NB100-236) is a polyclonal antibody validated for use in IHC and WB. Anti-Glucose 6 Phosphate Dehydrogenase Antibody: Cited in 18 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free
Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236]
Glucose-6-Phosphate-Dehydrogenase-Antibody-Western-Blot-NB100-236-img0009.jpgWestern Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236]
Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236] - Detection of Human and Mouse G6PD by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G6PD antibody NB100-236 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236] -
Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236] - Dysregulation of Antioxidant proteins in response to chronic endurance exercise in aged Nrf2−/− mice hearts. Antioxidant protein expression (GPX1, CAT, G6PD, & SOD1) were determined using immunoblotting with specific antibodies. The relative intensity signals were quantified using ImageJ software & normalized to GAPDH intensity & represented as histogram. Experiments were analyzed using one way ANOVA followed by Tukey multiple comparison tests & differences between the means were considered statistically significant if P < 0.05 (* vs. WT-SED, # vs. Nrf2−/−-SED, $ vs. WT CEE). Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/article/10.3389/fphys.2017.00268/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry-Frozen
1:10-1:500
Western Blot
1:500-1:2500
Application Notes
Use in IHC-Frozen reported in scientific literature (PMID: 17693254). Use in Immunoblotting reported in scientific literature (PMID 28515695).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: G6PD
Long Name
Glucose-6-Phosphate Dehydrogenase
Alternate Names
G6PDH
Gene Symbol
G6PD
Additional G6PD Products
Product Documents for Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free
Product Specific Notices for Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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