Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-236
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunoblotting
Cited:
Immunohistochemistry-Frozen, Western Blot, Immunoblotting
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residues 50 and 100 of human Glucose-6-Phosphate Dehydrogenase using the numbering given in entry NP_000393.2 (GeneID 2539).
Marker
Cytosol Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free
Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236]
Glucose-6-Phosphate-Dehydrogenase-Antibody-Western-Blot-NB100-236-img0009.jpgWestern Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236]
Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236] - Detection of Human and Mouse G6PD by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G6PD antibody NB100-236 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236] -
Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236] - Dysregulation of Antioxidant proteins in response to chronic endurance exercise in aged Nrf2−/− mice hearts. Antioxidant protein expression (GPX1, CAT, G6PD, & SOD1) were determined using immunoblotting with specific antibodies. The relative intensity signals were quantified using ImageJ software & normalized to GAPDH intensity & represented as histogram. Experiments were analyzed using one way ANOVA followed by Tukey multiple comparison tests & differences between the means were considered statistically significant if P < 0.05 (* vs. WT-SED, # vs. Nrf2−/−-SED, $ vs. WT CEE). Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/article/10.3389/fphys.2017.00268/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry-Frozen
1:10-1:500
Western Blot
1:500-1:2500
Application Notes
Use in IHC-Frozen reported in scientific literature (PMID: 17693254). Use in Immunoblotting reported in scientific literature (PMID 28515695).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: G6PD
Long Name
Glucose-6-Phosphate Dehydrogenase
Alternate Names
G6PDH
Gene Symbol
G6PD
Additional G6PD Products
Product Documents for Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free
Product Specific Notices for Glucose 6 Phosphate Dehydrogenase Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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