GLP-1R Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97308
![Knockdown Validated: GLP-1R Antibody - BSA Free [NBP1-97308]](https://resources.bio-techne.com/images/products/GLP-1R-Antibody-Western-Blot-NBP1-97308-img0015.jpg "Western Blot: GLP-1R Antibody - BSA Free [NBP1-97308]")
![Western Blot: GLP-1R AntibodyBSA Free [NBP1-97308]](https://resources.bio-techne.com/images/products/GLP-1R-Antibody-Western-Blot-NBP1-97308-img0014.jpg "Western Blot: GLP-1R AntibodyBSA Free [NBP1-97308]")
![Immunohistochemistry: GLP-1R Antibody - BSA Free [NBP1-97308]](https://resources.bio-techne.com/images/products/GLP-1R-Antibody-Immunohistochemistry-NBP1-97308-img0009.jpg "Immunohistochemistry: GLP-1R Antibody - BSA Free [NBP1-97308]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Validated by
Knockout/Knockdown, Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat, Canine
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Dot Blot, Knockdown Validated
Cited:
Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Cytometric Bead Assay Standard, IF/IHC, Knockdown Validated
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A synthetic peptide made to an internal portion of the human GLP1R protein (between residues 250-350) [UniProt P43220]
Reactivity Notes
Rat reactivity reported in scientific literature (PMID: 27435156). Canine reactivity reported in scientific literature (PMID: 25747753).
Localization
Cell membrane; Multi-pass membrane protein.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for GLP-1R Antibody - BSA Free
Immunohistochemistry: GLP-1R Antibody - BSA Free [NBP1-97308]
Immunohistochemistry: GLP-1R Antibody [NBP1-97308] - Analysis of GLP1R in mouse pancreas using DAB with hematoxylin counterstain.![Simple Western: GLP-1R AntibodyBSA Free [NBP1-97308]](https://resources.bio-techne.com/images/products/GLP-1R-Antibody-Simple-Western-NBP1-97308-img0006.jpg "Simple Western: GLP-1R AntibodyBSA Free [NBP1-97308]")
Simple Western: GLP-1R AntibodyBSA Free [NBP1-97308]
Simple Western: GLP-1R Antibody [NBP1-97308] - Simple Western lane view shows a specific band for GLP-1R in 0.5 mg/ml of Human Pancreas (left) and Mouse Pancreas (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody![Western Blot: GLP-1R AntibodyBSA Free [NBP1-97308]](https://resources.bio-techne.com/images/products/GLP-1R-Antibody-Western-Blot-NBP1-97308-img0016.jpg "Western Blot: GLP-1R AntibodyBSA Free [NBP1-97308]")
![Immunocytochemistry/ Immunofluorescence: GLP-1R Antibody - BSA Free [NBP1-97308]](https://resources.bio-techne.com/images/products/GLP-1R-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-97308-img0004.jpg "Immunocytochemistry/ Immunofluorescence: GLP-1R Antibody - BSA Free [NBP1-97308]")
![Immunohistochemistry-Paraffin: GLP-1R Antibody - BSA Free [NBP1-97308]](https://resources.bio-techne.com/images/products/GLP-1R-Antibody-Immunohistochemistry-Paraffin-NBP1-97308-img0011.jpg "Immunohistochemistry-Paraffin: GLP-1R Antibody - BSA Free [NBP1-97308]")
![Western Blot: GLP-1R AntibodyBSA Free [NBP1-97308]](https://resources.bio-techne.com/images/products/GLP-1R-Antibody-Western-Blot-NBP1-97308-img0008.jpg "Western Blot: GLP-1R AntibodyBSA Free [NBP1-97308]")
![Immunocytochemistry/ Immunofluorescence: GLP-1R Antibody - BSA Free [NBP1-97308]](https://resources.bio-techne.com/images/products/GLP-1R-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-97308-img0012.jpg "Immunocytochemistry/ Immunofluorescence: GLP-1R Antibody - BSA Free [NBP1-97308]")
![Immunohistochemistry-Paraffin: GLP-1R Antibody - BSA Free [NBP1-97308]](https://resources.bio-techne.com/images/products/GLP-1R-Antibody-Immunohistochemistry-Paraffin-NBP1-97308-img0010.jpg "Immunohistochemistry-Paraffin: GLP-1R Antibody - BSA Free [NBP1-97308]")
Applications for GLP-1R Antibody - BSA Free
Application
Recommended Usage
Dot Blot
reported in scientific literature (PMID 27435156)
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:200
Immunohistochemistry-Paraffin
1:200 - 1:400
Knockdown Validated
reported in scientific literature (PMID 31900217)
Simple Western
1:100
Western Blot
1:1000
Application Notes
In WB, a band is seen ~53kDa representing GLP1R. In ICC/IF, membrane staining was observed in HeLa cells. In IHC-P, strong membrane staining was observed in mouse pancreas tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in Human Pancreas and Mouse Pancreas lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:100. Separated by Size-Wes, Sally Sue/Peggy Sue.
See Simple Western Antibody Database for Simple Western validation: Tested in Human Pancreas and Mouse Pancreas lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:100. Separated by Size-Wes, Sally Sue/Peggy Sue.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GLP-1R
Long Name
Glucagon-like Peptide 1 Receptor
Alternate Names
GLP1R
Entrez Gene IDs
2740 (Human)
Gene Symbol
GLP1R
UniProt
Additional GLP-1R Products
Product Documents for GLP-1R Antibody - BSA Free
Product Specific Notices for GLP-1R Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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