GLI-3 Antibody (24GB6105)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51009

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NBP3-51009-100ul
NBP3-51009-20ul
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB6105

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all GLI-3 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human GLI-3 (Uniprot: P10071)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GLI-3 Antibody (24GB6105)

GLI-3 Antibody (24GB6105)

Immunocytochemistry/ Immunofluorescence: GLI-3 Antibody (24GB6105)[NBP3-51009] -

Immunocytochemical staining of HepG2 cells with GLI-3 antibody (1:1,000). Nuclei were stained blue with DAPI; Gli3 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
GLI-3 Antibody (24GB6105)

Flow Cytometry: GLI-3 Antibody (24GB6105)[NBP3-51009] -

Flow cytometric analysis of GLI-3expression in HepG2 cells using GLI-3 antibody (1:2,000). Green, isotype control; red, gli3.
GLI-3 Antibody (24GB6105)

Western Blot: GLI-3 Antibody (24GB6105)[NBP3-51009] -

Western blotting analysis using GLI-3 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit (GLI Family Zinc Finger 3.
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Applications for GLI-3 Antibody (24GB6105)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GLI-3

Gli-3 (also known as Zinc Finger Protein Gli-3 or GLI-Kruppel family member GLI-3) belongs to the GLI C2H2-type zinc-finger protein family and contains 5 C2H2-type zinc fingers. Gli-3 is very important for normal limb and brain development and is implicated in the transduction of Shh signal. Gli-3 is a nuclear protein expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium. Defects in Gli-3 are the cause of Greig cephalo-polysyndactyly syndrome (GCPS); an autosomal dominant disorder-affecting limb and craniofacial development. Two isoforms of human Gli-3 have been reported. One is the fulllength protein at ~170-190kDa and the other is a truncated isoform at ~80kDa.

Long Name

GLI-Kruppel family member GLI3

Alternate Names

ACLS, ADD, Bph, GCPS, GLI3, PAPA1, PAPB, Pdn, PHS, PPDIV, Xt

Gene Symbol

GLI3

Additional GLI-3 Products

Product Documents for GLI-3 Antibody (24GB6105)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GLI-3 Antibody (24GB6105)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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