GLI-1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-68877
![Immunocytochemistry/ Immunofluorescence: GLI-1 Antibody [NBP2-68877]](https://resources.bio-techne.com/images/products/GLI-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-68877-img0001.jpg "Immunocytochemistry/ Immunofluorescence: GLI-1 Antibody [NBP2-68877]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: PCLDFDSPTHSTGQLKAQLVCNYVQSQQELLWEGGGREDAPAQEPSYQSPKFLGGSQVSPSRAKAPVNTYGPGFGPNLPNHKSGSYP
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for GLI-1 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: GLI-1 Antibody [NBP2-68877]
Immunocytochemistry/Immunofluorescence: GLI-1 Antibody [NBP2-68877] - Staining of human cell line RH-30 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
Chromatin Immunoprecipitation ChIP: GLI-1 Antibody - BSA Free
ChIP-Exo-Seq composite graph for Anti-GLI-1 tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh's Lab at Cornell University.Applications for GLI-1 Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation-exo-Seq
1-10ug per reaction
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Application Notes
ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GLI-1
Long Name
Glioma-Associated Oncogene Homolog 1 [Zinc Finger Protein]
Alternate Names
GLI1
Gene Symbol
GLI1
Additional GLI-1 Products
Product Documents for GLI-1 Antibody - BSA Free
Product Specific Notices for GLI-1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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