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GL7 (T and B Cell Activation Marker) Antibody (GL-7)

Catalog # NBP2-00362 | Novus Biologicals a Bio-Techne Brand
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NBP2-00362-0.025mg
NBP2-00362-100ug
NBP2-00362-500ug

Key Product Details

Species Reactivity

Human, Mouse

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Rat IgM Clone # GL-7

Concentration

0.5 mg/ml

Product Summary for GL7 (T and B Cell Activation Marker) Antibody (GL-7)

Immunogen

The immunogen for this antibody is T- and B-cell activation antigen (GL7, Ly-77).

Clonality

Monoclonal

Host

Rat

Isotype

IgM

Scientific Data Images for GL7 (T and B Cell Activation Marker) Antibody (GL-7)

Flow Cytometry: GL7 (T and B Cell Activation Marker) Antibody (GL-7) [NBP2-00362] - Analysis using the Biotin conjugate of NBP2-00362. Staining of 3-day unstimulated (left) and 3-day ConA activated (right) BALB/c splenocytes with 0.5 ug of Rat IgM Isotype Control Biotin (open histogram) or 0.25ug of Anti-Human/Mouse GL7 (T and B Cell Activation Marker) Biotin (filled histogram) followed by Streptavidin PE. Total viable cells were used for analysis.
Flow Cytometry: GL7 (T and B Cell Activation Marker) Antibody (GL-7) [NBP2-00362] - Staining of 3-day unstimulated (left) and 3-day ConA-stimulated (right) BALB/c splenocytes with 0.25 ug of Rat IgM kappa Isotype Control Purified (open histogram) or 0.25 ug of Anti-Human/Mouse GL7 (T and B Cell Activation Marker) Purified (filled histogram) followed by Anti-Rat IgM PE.

Applications for GL7 (T and B Cell Activation Marker) Antibody (GL-7)

Application
Recommended Usage

Flow Cytometry

< = 0.5 ug/10^6 cells in 100 ul

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500
Application Notes
The GL-7 (GL7) antibody has been tested by flow cytometric analysis of ConA-activated mouse splenocytes. This can be used at less than or equal to 0.5 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The GL-7 antibody has also been reported for use in immunoprecipitation and immunohistochemical staining of frozen tissue sections. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2)

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: GL7 (T and B Cell Activation Marker)

This GL7 monoclonal antibody reacts with a cell-surface protein found on T and B lymphocytes activated in vitro, on bone marrow pre-BII cells, germinal center B cells, and also human B cell lines Ramos and Daudi. There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells, with expression in BALB/c greater than that in C57BL/6. GL7 is commonly used as a marker for mouse germinal center B cells. The epitope of GL7 has been identified as a sialic acid glycan moiety called Neu5Ac. This moiety is recognized by CD22.

Product Documents for GL7 (T and B Cell Activation Marker) Antibody (GL-7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GL7 (T and B Cell Activation Marker) Antibody (GL-7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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