Girdin Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-71857
![Western Blot: Girdin Antibody [NBP1-71857]](https://resources.bio-techne.com/images/products/Girdin-Antibody-Western-Blot-NBP1-71857-img0003.jpg "Western Blot: Girdin Antibody [NBP1-71857]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%). Backed by our 100% Guarantee.
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 1425 and 1475 of human Coiled-Coil Domain Containing 88A using the numbering given in entry NP_001129069.1 (GeneID 55704).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit Girdin Antibody - BSA Free (NBP1-71857) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Girdin Antibody - BSA Free
Western Blot: Girdin Antibody [NBP1-71857]
Western Blot: Girdin Antibody [NBP1-71857] - Detection of Human CCDC88A by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CCDC88A antibody NBP1-71857 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.![Western Blot: Girdin Antibody [NBP1-71857]](https://resources.bio-techne.com/images/products/Girdin-Antibody-Western-Blot-NBP1-71857-img0001.jpg "Western Blot: Girdin Antibody [NBP1-71857]")
Western Blot: Girdin Antibody [NBP1-71857]
Western Blot: Girdin Antibody [NBP1-71857] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-CCDC88A antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. CCDC88A was also immunoprecipitated by rabbit anti-CCDC88A antibody NBP1-71856 , which recognizes an upstream epitope. For blotting immunoprecipitated CCDC88A, NBP1-71857 was used at 1 ug/ml.Applications for Girdin Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:2000-1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Girdin
Girdin (GIRDers of actIN filaments; also APE and GIV) is a member of the CCDC88 family of molecules. It is a cytosolic protein, ubiquitously expressed, and plays a key role in the motility of breast cancer cells. Human Girdin is 1871 amino acids (aa) in length. It contains two coiled-coil regions that mediate homodimerization (aa 196-425 and 458-1358), and one phosphoinositide-binding domain (aa 1390-1408). Normally, Girdin is membrane-associated via an interaction with phosphoinositides. Upon phosphorylation at Ser1417 by Akt, it relocates to lamellipodia where it crosslinks actin filaments. Multiple isoforms of Girdin have been detected.
Long Name
Girders of Actin Filament
Alternate Names
APE, CCDC88A, GIV, GRDN, HkRP1
Gene Symbol
CCDC88A
UniProt
Additional Girdin Products
Product Documents for Girdin Antibody - BSA Free
Product Specific Notices for Girdin Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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