Girdin Antibody (M012)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23351
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # M012
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Clone (M012) was generated from a recombinant protein that included amino acid residues within the C-terminal region of human Girdin. [UniProt# Q3V6T2]
Specificity
Clone M012 mouse monoclonal antibody detects a 250 kDa protein on SDS-PAGE immunoblots of human A431 cells and human brain tissue. The antibody also works for immmunoprecipitation, immunohistochemistry, and immunocytochemistry.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
250 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for Girdin Antibody (M012)
Western Blot: Girdin Antibody (M012) [NBP3-23351] -
Western Blot: Girdin Antibody (M012) [NBP3-23351] - Western blot image of human A431 cell lysates treated with pervanadate (lanes 1-4). The blot was treated with alkaline phosphatase to dephosphorylate Girdin phosphosites (lanes 2 & 4). The blot was probed with mouse monoclonal anti-Girdin (lanes 1 & 2) or rabbit polyclonal anti-Girdin (Tyr-1764), phospho-specific (lanes 3 & 4).
Immunocytochemistry/Immunofluorescence: Girdin Antibody (M012) [NBP3-23351] -
Immunocytochemistry/Immunofluorescence: Girdin Antibody (M012) [NBP3-23351] - Immunocytochemical labeling of Girdin in aldehyde fixed and NP-40 permeabilized human NCI-H1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-Girdin (NBP3-23351). The antibody was detected using goat anti-mouse DyLight(R) 594.Applications for Girdin Antibody (M012)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50
Immunohistochemistry
1:50
Immunoprecipitation
1:50
Western Blot
1:250
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: Girdin
Girdin (GIRDers of actIN filaments; also APE and GIV) is a member of the CCDC88 family of molecules. It is a cytosolic protein, ubiquitously expressed, and plays a key role in the motility of breast cancer cells. Human Girdin is 1871 amino acids (aa) in length. It contains two coiled-coil regions that mediate homodimerization (aa 196-425 and 458-1358), and one phosphoinositide-binding domain (aa 1390-1408). Normally, Girdin is membrane-associated via an interaction with phosphoinositides. Upon phosphorylation at Ser1417 by Akt, it relocates to lamellipodia where it crosslinks actin filaments. Multiple isoforms of Girdin have been detected.
Long Name
Girders of Actin Filament
Alternate Names
APE, CCDC88A, GIV, GRDN, HkRP1
Gene Symbol
CCDC88A
Additional Girdin Products
Product Documents for Girdin Antibody (M012)
Product Specific Notices for Girdin Antibody (M012)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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