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GFPT2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-56688

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NBP1-56688

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Synthetic peptides corresponding to GFPT2(glutamine-fructose-6-phosphate transaminase 2) The peptide sequence was selected from the middle region of GFPT2 (NP_005101). Peptide sequence TARQGRPIILCSKDDTESSKFAYKTIELPHTVDCLQGILSVIPLQLLSFH. The peptide sequence for this immunogen was taken from within the described region.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 23407165).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

77 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for GFPT2 Antibody - BSA Free

Western Blot: GFPT2 Antibody [NBP1-56688]

Western Blot: GFPT2 Antibody [NBP1-56688]

Western Blot: GFPT2 Antibody [NBP1-56688] - HT1080 cell lysate, concentration 0.2-1 ug/ml.
GFPT2 Antibody - BSA Free

Western Blot: GFPT2 Antibody - BSA Free [NBP1-56688] -

Regulation of putative ADO substrates human cells.a, b WT, ADO KO or ADO overexpressing (OE) SH-SY5Y (a) or U87-MG (b) were exposed to hypoxia (1% O2) for 16 h, then protein samples were collected and analysed for the expression of various potential ADO substrates. RGS4 and RGS5 were used as positive controls. c HEK 293T cells were transiently co-transfected with cDNAs encoding ANKRD29 or SUSD6, alongside either CDO1 or ADO, and subjected to hypoxia as before. d, e SH-SY5Y cells stably expressing either ANKRD29:FLAG (d) or SUSD6:FLAG (e) were subjected to hypoxia for 16 h and the expression of both proteins was analysed using an anti-FLAG antibody. RGS5 expression was also analysed as a positive control. SUSD6 was detected at a much higher level relative to RGS5 and ANKRD29 in a stably polyclonal population. All blots (a–e) are representative of at least 3 independent experiments. Source data (including relevant molecular weight markers) are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41467-024-49489-2), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for GFPT2 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GFPT2

GFPT2 controls the flux of glucose into the hexosamine pathway. GFPT2 is most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins.

Alternate Names

D-fructose-6-phosphate amidotransferase 2, FLJ10380, GFAT 2, GFAT2EC 2.6.1.16, glucosamine--fructose-6-phosphate aminotransferase [isomerizing] 2, glutamine: fructose-6-phosphate aminotransferase 2, Glutamine:fructose 6 phosphate amidotransferase 2, glutamine-fructose-6-phosphate transaminase 2, Hexosephosphate aminotransferase 2

Entrez Gene IDs

9945 (Human)

Gene Symbol

GFPT2

UniProt

Additional GFPT2 Products

Product Documents for GFPT2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for GFPT2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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