GFPT2 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-56688
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Synthetic peptides corresponding to GFPT2(glutamine-fructose-6-phosphate transaminase 2) The peptide sequence was selected from the middle region of GFPT2 (NP_005101). Peptide sequence TARQGRPIILCSKDDTESSKFAYKTIELPHTVDCLQGILSVIPLQLLSFH. The peptide sequence for this immunogen was taken from within the described region.
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 23407165).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
77 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Scientific Data Images for GFPT2 Antibody - BSA Free
Western Blot: GFPT2 Antibody [NBP1-56688]
Western Blot: GFPT2 Antibody [NBP1-56688] - HT1080 cell lysate, concentration 0.2-1 ug/ml.Western Blot: GFPT2 Antibody - BSA Free [NBP1-56688] -
Regulation of putative ADO substrates human cells.a, b WT, ADO KO or ADO overexpressing (OE) SH-SY5Y (a) or U87-MG (b) were exposed to hypoxia (1% O2) for 16 h, then protein samples were collected and analysed for the expression of various potential ADO substrates. RGS4 and RGS5 were used as positive controls. c HEK 293T cells were transiently co-transfected with cDNAs encoding ANKRD29 or SUSD6, alongside either CDO1 or ADO, and subjected to hypoxia as before. d, e SH-SY5Y cells stably expressing either ANKRD29:FLAG (d) or SUSD6:FLAG (e) were subjected to hypoxia for 16 h and the expression of both proteins was analysed using an anti-FLAG antibody. RGS5 expression was also analysed as a positive control. SUSD6 was detected at a much higher level relative to RGS5 and ANKRD29 in a stably polyclonal population. All blots (a–e) are representative of at least 3 independent experiments. Source data (including relevant molecular weight markers) are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41467-024-49489-2), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for GFPT2 Antibody - BSA Free
Application
Recommended Usage
Western Blot
1.0 ug/ml
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 2% Sucrose
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GFPT2
Alternate Names
D-fructose-6-phosphate amidotransferase 2, FLJ10380, GFAT 2, GFAT2EC 2.6.1.16, glucosamine--fructose-6-phosphate aminotransferase [isomerizing] 2, glutamine: fructose-6-phosphate aminotransferase 2, Glutamine:fructose 6 phosphate amidotransferase 2, glutamine-fructose-6-phosphate transaminase 2, Hexosephosphate aminotransferase 2
Entrez Gene IDs
9945 (Human)
Gene Symbol
GFPT2
UniProt
Additional GFPT2 Products
Product Specific Notices for GFPT2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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