GCS1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14967
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated
Cited:
Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human GCS1. The exact sequence is proprietary.
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 29465311).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for GCS1 Antibody - BSA Free
![Western Blot: GCS1 Antibody [NBP2-14967]](https://resources.bio-techne.com/images/products/GCS1-Antibody-Western-Blot-NBP2-14967-img0001.jpg "Western Blot: GCS1 Antibody [NBP2-14967]")
Western Blot: GCS1 Antibody [NBP2-14967]
Western Blot: GCS1 Antibody [NBP2-14967] - (30 ug whole cell lysate) H12997. 5% SDS PAGE, antibody diluted at 1:1000.![Western Blot: GCS1 Antibody [NBP2-14967]](https://resources.bio-techne.com/images/products/GCS1-Antibody-Western-Blot-NBP2-14967-img0002.jpg "Western Blot: GCS1 Antibody [NBP2-14967]")
Western Blot: GCS1 Antibody [NBP2-14967]
Western Blot: GCS1 Antibody [NBP2-14967] - analysis of GCS1 in (L1) 100 ug of WT MEF cells and (L2) MEFs treated with GCS1 specific siRNA for 48 hours using anti-GCS1 antibody. * indicates the non-specific band recognized by the antibody. Image from verified customer review.![Immunoprecipitation: GCS1 Antibody [NBP2-14967]](https://resources.bio-techne.com/images/products/GCS1-Antibody-Immunoprecipitation-NBP2-14967-img0007.jpg "Immunoprecipitation: GCS1 Antibody [NBP2-14967]")
Immunoprecipitation: GCS1 Antibody [NBP2-14967]
Immunoprecipitation: GCS1 Antibody [NBP2-14967] - Immunoprecipitation was performed by using 3ug of [NBP2-14967] in 2 mg whole cell lysate from WT MEF cells. The precipitated proteins were enriched with Protein A beads. The eluted proteins were analyzed through 12.5% SDS PAGE. Arrow indicates GCS1 band. Image from verified customer review.Applications for GCS1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Reported in scientific literature (PMID:29465311)
Immunoprecipitation
Validated from a verified customer review.
Knockdown Validated
Reported in scientific literature (PMID:29465311)
Western Blot
1:500-1:3000
Reviewed Applications
Read 2 reviews rated 4.5 using NBP2-14967 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol
Format
BSA Free
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: GCS1
Long Name
Mannosyl-oligosaccharide glucosidase
Alternate Names
EC 3.2.1.106, MOGS
Gene Symbol
MOGS
UniProt
Additional GCS1 Products
Product Documents for GCS1 Antibody - BSA Free
Product Specific Notices for GCS1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.