gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12540
![Immunoprecipitation: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540]](https://resources.bio-techne.com/images/products/gamma-Glutamylcyclotransferase-CRF21-GGCT-Antibody-Immunoprecipitation-NBP3-12540-img0004.jpg "Immunoprecipitation: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540]")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with purified, recombinant Human gamma-Glutamylcyclotransferase/CRF21/GGCT (Accession#: O75223-1; Met1-Leu188)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Scientific Data Images
![Western Blot: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540]](https://resources.bio-techne.com/images/products/gamma-Glutamylcyclotransferase-CRF21-GGCT-Antibody-Western-Blot-NBP3-12540-img0005.jpg "Western Blot: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540]")
Western Blot: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540]
Western Blot: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540] - Lane A: 293 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:21 kDaObserved band size:21 kDa(We are unsure as to the identity of these extra bands.)![Immunohistochemistry-Paraffin: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540]](https://resources.bio-techne.com/images/products/gamma-Glutamylcyclotransferase-CRF21-GGCT-Antibody-Immunohistochemistry-Paraffin-NBP3-12540-img0003.jpg "Immunohistochemistry-Paraffin: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540]")
Immunohistochemistry-Paraffin: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540]
Immunohistochemistry-Paraffin: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540] - Staining of human GGCT in human corpus uteri with rabbit polyclonal antibody (1:10000).![Immunohistochemistry-Paraffin: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540]](https://resources.bio-techne.com/images/products/gamma-Glutamylcyclotransferase-CRF21-GGCT-Antibody-Immunohistochemistry-Paraffin-NBP3-12540-img0002.jpg "Immunohistochemistry-Paraffin: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540]")
Immunohistochemistry-Paraffin: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540]
Immunohistochemistry-Paraffin: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540] - Staining of human GGCT in human hepatoma with rabbit polyclonal antibody (1:10000).Applications
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunohistochemistry
1:2500-1:10000
Immunohistochemistry-Paraffin
1:2500-1:10000
Immunoprecipitation
1-4 uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: gamma-Glutamylcyclotransferase/CRF21
CRF21 (Cytochrome c-releasing factor 21; also C7orf24 or LOC79017) is a 21 kDa AIG2-like family member. It is a cytosolic protein that induces the release of Cytochrome c from mitochondria, possibly initiating apoptosis. Human CRF21 is 188 amino acids (aa) in length and is a complex of beta-sheets and alpha-helices that create two terminal domains. It has been proposed that CRF21 forms homodimers. There are at least two possible splice variants of human CRF21, one showing a 70 aa substitution, while the second variant shows an 18 aa substitution for aa 97-188. Full-length human CRF21 is 82% aa identical to mouse CRF21.
Long Name
Cytochrome c Releasing Factor 21
Alternate Names
C7orf24, CRF21, gammaGlutamylcyclotransferase, GCTG, GGC, GGCT, LOC79017
Gene Symbol
GGCT
Additional gamma-Glutamylcyclotransferase/CRF21 Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...