Galectin-9 Antibody (RG9-35) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-09032
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Mouse
Applications
Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Functional Assay
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # RG9-35
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody was raised by immunising rats with recombinant mouse galectin-9.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for Galectin-9 Antibody (RG9-35) - Chimeric - Azide and BSA Free
Flow Cytometry: Galectin-9 Antibody (RG9-35) - Chimeric - Azide and BSA Free [NBP3-09032] -
Flow Cytometry: Galectin-9 Antibody (RG9-35) - Chimeric - Azide and BSA Free [NBP3-09032] - NIH3T3 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-09032 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Immunocytochemistry /Immunofluorescence: Galectin-9 Antibody (RG9-35) - Chimeric - Azide and BSA Free [NBP3-09032] -
Immunocytochemistry /Immunofluorescence: Galectin-9 Antibody (RG9-35) - Chimeric - Azide and BSA Free [NBP3-09032] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with NBP3-09032 at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom: NBP3-09032, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.Applications for Galectin-9 Antibody (RG9-35) - Chimeric - Azide and BSA Free
Application
Recommended Usage
Block/Neutralize
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Functional Assay
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Galectin-9
Alternate Names
Ecalectin, GAL9, Galectin9, LGALS9
Gene Symbol
LGALS9
Additional Galectin-9 Products
Product Documents for Galectin-9 Antibody (RG9-35) - Chimeric - Azide and BSA Free
Product Specific Notices for Galectin-9 Antibody (RG9-35) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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