Frequenin Antibody (24GB25)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51412
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 24GB25
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human Frequenin (Uniprot: P62166)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Frequenin Antibody (24GB25)
Immunocytochemistry/ Immunofluorescence: Frequenin Antibody (24GB25)[NBP3-51412] -
Frequenin antibody (1:1,000), Top panel: wild-type (WT); Bottom panal: neuronal calcium sensor 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Neuronal calcium sensor 1 was stained magenta with Alexa Fluor(R) 647. Scale bar, 20 um.
Immunocytochemistry/ Immunofluorescence: Frequenin Antibody (24GB25)[NBP3-51412] -
Immunocytochemical staining of HepG2 cells with Frequenin antibody (1:1,000). Nuclei were stained blue with DAPI; Neuronal calcium sensor 1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 um.
Flow Cytometry: Frequenin Antibody (24GB25)[NBP3-51412] -
Validation of Frequenin knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Frequenin antibody (1:2,000) and analyzed using BD flow cytometer.Applications for Frequenin Antibody (24GB25)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Frequenin
Alternate Names
DKFZp761L1223, FREQFLUP, frequenin (Drosophila) homolog, Frequenin homolog, frequenin homolog (Drosophila), Frequenin-like protein, Frequenin-like ubiquitous protein, NCS-1, neuronal calcium sensor 1
Gene Symbol
NCS1
Additional Frequenin Products
Product Documents for Frequenin Antibody (24GB25)
Product Specific Notices for Frequenin Antibody (24GB25)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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