FoxO1/FKHR Antibody (SU33-01)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67463
Recombinant Monoclonal Antibody.
![Immunocytochemistry/ Immunofluorescence: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463]](https://resources.bio-techne.com/images/products/FoxO1-FKHR-Antibody-SU33-01-Immunocytochemistry-Immunofluorescence-NBP2-67463-img0001.jpg "Immunocytochemistry/ Immunofluorescence: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Zebrafish
Cited:
Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # SU33-01 expressed in HEK293
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human FoxO1/FKHR aa 301-350 / 655. (SwissProt: Q12778 Human; SwissProt: Q9R1E0 Mouse; SwissProt: G3V7R4 Rat)
Localization
Cytoplasm, Nucleus.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for FoxO1/FKHR Antibody (SU33-01)
![Western Blot: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463]](https://resources.bio-techne.com/images/products/FoxO1-FKHR-Antibody-SU33-01-Western-Blot-NBP2-67463-img0008.jpg "Western Blot: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463]")
Western Blot: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463]
Western Blot: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Analysis of FOXO1A on different lysates with Rabbit anti-FOXO1A antibody at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: NIH/3T3 cell lysate ysates/proteins at 10 ug/Lane. Predicted band size: 70 kDa observed band size: 85 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.![Immunocytochemistry/ Immunofluorescence: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463]](https://resources.bio-techne.com/images/products/FoxO1-FKHR-Antibody-SU33-01-Immunocytochemistry-Immunofluorescence-NBP2-67463-img0003.jpg "Immunocytochemistry/ Immunofluorescence: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463]")
Immunocytochemistry/ Immunofluorescence: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463]
Immunocytochemistry/Immunofluorescence: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Staining FOXO1A in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.![Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463]](https://resources.bio-techne.com/images/products/FoxO1-FKHR-Antibody-SU33-01-Immunohistochemistry-Paraffin-NBP2-67463-img0007.jpg "Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463]")
Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463]
Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Analysis of paraffin-embedded mouse heart tissue using anti-FOXO1A antibody. Counter stained with hematoxylin.![Immunocytochemistry/ Immunofluorescence: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463]](https://resources.bio-techne.com/images/products/FoxO1-FKHR-Antibody-SU33-01-Immunocytochemistry-Immunofluorescence-NBP2-67463-img0002.jpg "Immunocytochemistry/ Immunofluorescence: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463]")
![Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463]](https://resources.bio-techne.com/images/products/FoxO1-FKHR-Antibody-SU33-01-Immunohistochemistry-Paraffin-NBP2-67463-img0004.jpg "Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463]")
![Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463]](https://resources.bio-techne.com/images/products/FoxO1-FKHR-Antibody-SU33-01-Immunohistochemistry-Paraffin-NBP2-67463-img0005.jpg "Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463]")
![Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463]](https://resources.bio-techne.com/images/products/FoxO1-FKHR-Antibody-SU33-01-Immunohistochemistry-Paraffin-NBP2-67463-img0006.jpg "Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463]")
Applications for FoxO1/FKHR Antibody (SU33-01)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:1000-1:2000
Application Notes
IHC-Fr usage was reported in scientific literature (PMID: 31196960).
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: FoxO1/FKHR
FoxO1, also called FKHR (forkhead in rhabdomyosarcoma), is a ubiquitously expressed member of the forkhead box O family of winged helix transcription factors. In neurons, it is activated in response to stress, translocating to the nucleus where it promotes apoptosis and blocks proliferation. In insulin-responsive tissues, nutrient abundance triggers phosphorylation by AKT that blocks nuclear translocation and activity.
Long Name
Forkhead Box O1
Alternate Names
FKH1, FKHR
Gene Symbol
FOXO1
Additional FoxO1/FKHR Products
Product Documents for FoxO1/FKHR Antibody (SU33-01)
Product Specific Notices for FoxO1/FKHR Antibody (SU33-01)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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